Gao Xin Qi, Wang Xiu Ling
Department of Biology, Qufu Normal University, Shandong Qufu 273165.
Shi Yan Sheng Wu Xue Bao. 2002 Mar;35(1):47-53.
Ultracytochemical localization of adenosine triphosphatase (ATPase) activity in stigmas, pollens and pollen tubes of Fagopyrum esculentum was performed with the cytochemical method of lead phosphate precipitation. The results were as follows: (1) Lower activities of ATPase appeared in stigma cells at 0.5 hour after compatible and incompatible pollination. Stigma surface and pollen grains attached on stigma showed higher ATPase activities after compatible pollination and lower or no activities after incompatible pollination at 0.5 hour. ATPase localized on endoplasmic reticula and sperm cell in pollen grain. (2) Lower ATPase activities appeared both in stigma cells and pollen tubes in style at 1.5 hours after incompatible pollination. Pollen tube stopped growing and the degeneration of its cytoplasma began; on the contrary, at 1.5 hours after compatible pollination, higher ATPase activities were detected both in stigma cells and pollen tube in style. ATPase localized mainly on plasmolemma, in cytoplasmic matrix of stigma cells and in mitochondria, dictyosome, plastid envelop, and on the wall of pollen tube as well. The present study indicated that the stop of pollen tube growth after self-incompatibility of Fagopyrum esculentum resulted not only from the nutrient starvation of pollen tube, but also from its metabolic disorder in incompatible style.
采用磷酸铅沉淀细胞化学方法，对荞麦柱头、花粉和花粉管中的三磷酸腺苷酶（ATPase）活性进行了超微细胞化学定位。结果如下：（1）在亲和与不亲和授粉后0.5小时，柱头细胞中ATPase活性较低。在亲和授粉后0.5小时，柱头表面和附着在柱头上的花粉粒显示出较高的ATPase活性，而在不亲和授粉后则显示较低或无活性。ATPase定位于花粉粒中的内质网和精细胞上。（2）在不亲和授粉后1.5小时，柱头细胞和花柱中的花粉管中ATPase活性均较低。花粉管停止生长，其细胞质开始退化；相反，在亲和授粉后1.5小时，柱头细胞和花柱中的花粉管中均检测到较高的ATPase活性。ATPase主要定位于质膜上、柱头细胞的细胞质基质中、线粒体、高尔基体、质体包膜以及花粉管壁上。本研究表明，荞麦自交不亲和后花粉管生长的停止不仅是由于花粉管营养饥饿，还由于其在不亲和花柱中的代谢紊乱。
