Qin Yuan, Chen Dan, Zhao Jie
Key Laboratory of Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao. 2006 Jun;32(3):307-14.
The distribution patterns of arabinogalactan-proteins (AGPs) in stigma and style of Nicotiana tabacum L. were studied with western blotting, immunohistochemistry and ultracytochemistry techniques. The results showed abundant AGPs accumulated in the stigmas and styles (Fig.1, Plate I). AGPs were mainly distributed in the cytoplasm of epidermal cells and extracellular matrix of the secretory zone of stigma (Plate II) and no significant changes of the distribution of AGPs in stigma were detected before and after pollination. In styles, AGPs were mainly distributed in the outer cell walls of the epidermal cells, the cytoplasm of peripheral cells of vascular bundles and the extracellular matrix of the transmitting tissues (Plate III). As the pollen tube tips penetrated through, the accumulations of AGPs in the extracellular matrix of the stylar transmitting tissues decreased, but abundant AGPs were founded in the cytoplasm and cell walls of the passing pollen tubes (Plate III).
采用蛋白质免疫印迹法、免疫组织化学和超微细胞化学技术，研究了阿拉伯半乳聚糖蛋白（AGPs）在烟草柱头和花柱中的分布模式。结果表明，AGPs在柱头和花柱中大量积累（图1，图版I）。AGPs主要分布在柱头分泌区表皮细胞的细胞质和细胞外基质中（图版II），授粉前后柱头中AGPs的分布无明显变化。在花柱中，AGPs主要分布在表皮细胞的外侧细胞壁、维管束周围细胞的细胞质以及传递组织的细胞外基质中（图版III）。随着花粉管尖端的穿透，花柱传递组织细胞外基质中AGPs的积累减少，但在穿过的花粉管的细胞质和细胞壁中发现大量AGPs（图版III）。
