Kozyrev D P, Vasinova N A
Tsitologiia. 2004;46(5):465-73.
An important component of nonspecific defence of human or animal organisms against microbial invasion is attempt to withhold growth-essential iron from invading bacteria. To overcome the barrier bacterial iron uptake systems is derepressed at low iron concentrations. In many pathogenic and nonpathogenic bacteria the regulation of the corresponding genes depends on the regulatory protein Fur. For cloning Fur-regulated loci, methods based on affinity of Fur to the corresponding promoter sites are used.
人类或动物机体针对微生物入侵的非特异性防御的一个重要组成部分，是试图阻止入侵细菌获取对其生长至关重要的铁。为了克服这一屏障，细菌的铁摄取系统在低铁浓度下会去阻遏。在许多致病性和非致病性细菌中，相应基因的调控取决于调节蛋白Fur。为了克隆Fur调控的基因座，人们使用基于Fur与相应启动子位点亲和力的方法。
