Ossipow Vincent, Pellissier François, Schaad Olivier, Ballivet Marc
Department of Biochemistry, University of Geneva, Sciences II, 1211 Geneva 4, Switzerland.
Mol Cell Neurosci. 2004 Sep;27(1):70-83. doi: 10.1016/j.mcn.2004.05.003.
The development of the primary visual cortex in animals possessing binocular vision is a classical paradigm for the study of activity-dependent neuronal plasticity. To elucidate the genetic determinants of this period of substantial plasticity, we conducted an unbiased and comprehensive transcript profiling analysis with differential display and DNA array techniques. We characterized the transcripts that change significantly between the critical and postcritical periods in the rat binocular visual cortex. We determined if these changes are specific for the visual cortex by simultaneously profiling the hippocampus and examined the impact of sensory experience on the accumulation of the identified transcripts. Our results uncover visual cortex-specific and unspecific transcription programs. Transcripts for protein kinases and phosphatases are particularly regulated. The identified transcripts support the notion that the critical period provides a permissive state for plasticity.
在具有双眼视觉的动物中，初级视觉皮层的发育是研究活动依赖性神经元可塑性的经典范例。为了阐明这一高度可塑性时期的基因决定因素，我们使用差异显示和DNA阵列技术进行了无偏且全面的转录本谱分析。我们对大鼠双眼视觉皮层关键期和关键期后显著变化的转录本进行了表征。我们通过同时分析海马体来确定这些变化是否对视觉皮层具有特异性，并研究了感觉经验对已鉴定转录本积累的影响。我们的结果揭示了视觉皮层特异性和非特异性的转录程序。蛋白激酶和磷酸酶的转录本受到特别调控。已鉴定的转录本支持了关键期为可塑性提供许可状态这一观点。
