Jiang Wen Shan, Yin Zheng Qin
Southwest Eye Hospital, Third Military Medical University, Chongqing, China.
Curr Eye Res. 2007 Jul-Aug;32(7-8):709-16. doi: 10.1080/02713680701473251.
To investigate the molecular mechanism involved in the termination of the critical period of visual cortex plasticity in the rat.
The rats were divided into two groups, one group was dark-reared for 67 days after birth, while the other group was dark-reared for 60 days and then put under a normal light/dark cycle for 7 days. A subtracted cDNA library was constructed from the visual cortex, and differentially expressed genes were screened by nested PCR, reverse Northern hybridization, sequencing, and homology analysis.
Fourteen genes were found to be up-regulated in the visual cortex. These included 13 known genes and a novel fragment (Genbank submission EB174193). Of the known genes, three genes encoding beta -tubulin, myelin basic protein, and cyclophilin were previously reported to be associated with visual cortex plasticity.
A set of candidate genes related to the termination of the critical period was identified using the subtracted cDNA library. This work provides an important basis for understanding the molecular mechanisms involved in the termination of plasticity in the visual cortex.
研究大鼠视觉皮层可塑性关键期终止所涉及的分子机制。
将大鼠分为两组，一组出生后黑暗饲养67天，另一组黑暗饲养60天，然后置于正常光/暗循环7天。从视觉皮层构建消减cDNA文库，并通过巢式PCR、反向Northern杂交、测序和同源性分析筛选差异表达基因。
发现视觉皮层中有14个基因上调。其中包括13个已知基因和一个新片段（已提交Genbank，登录号EB174193）。在已知基因中，先前报道编码β-微管蛋白、髓鞘碱性蛋白和亲环蛋白的三个基因与视觉皮层可塑性相关。
利用消减cDNA文库鉴定出一组与关键期终止相关的候选基因。这项工作为理解视觉皮层可塑性终止所涉及的分子机制提供了重要依据。
