Podgornik A, Jancar J, Merhar M, Kozamernik S, Glover D, Cucek K, Barut M, Strancar A
BIA Separations d.o.o., Teslova 30, SI-1000, Ljubljana, Slovenia.
J Biochem Biophys Methods. 2004 Sep 30;60(3):179-89. doi: 10.1016/j.jbbm.2004.01.002.
Monoliths represent a special class of chromatographic supports. In contrast to other stationary phases, they consist of a single piece of highly porous material through which a sample is mainly transported by convection. As a consequence, monoliths enable fast separations and exhibit flow-unaffected properties, which make them attractive for purification of macromolecules like proteins or DNA. In this work, methacrylate-based monolithic columns with the bed volume up to 8000 ml are characterized. They perform high-resolution separations of several hundreds of grams of proteins per hour by utilizing liter per minute flow rates. They are incompressible under these operating conditions and resistant to strong alkaline conditions.
整体柱代表了一类特殊的色谱载体。与其他固定相不同，它们由单一的高度多孔材料组成，样品主要通过对流在其中传输。因此，整体柱能够实现快速分离，并表现出不受流速影响的特性，这使得它们对于蛋白质或DNA等大分子的纯化具有吸引力。在这项工作中，对床体积高达8000毫升的基于甲基丙烯酸酯的整体柱进行了表征。它们通过使用每分钟升的流速，每小时能够对数百克蛋白质进行高分辨率分离。在这些操作条件下，它们不可压缩，并且耐强碱性条件。
