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本文引用的文献

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Molecular analysis of deep subsurface microbial communities in Nankai Trough sediments (ODP Leg 190, Site 1176).南黄海沉积物（ODP 第 190 航次，站位 1176）深部微生物群落的分子分析。
FEMS Microbiol Ecol. 2003 Jul 1;45(2):115-25. doi: 10.1016/S0168-6496(03)00128-4.
2
Diversity of prokaryotes and methanogenesis in deep subsurface sediments from the Nankai Trough, Ocean Drilling Program Leg 190.大洋钻探计划第190航次南海海槽深层地下沉积物中微生物的多样性及产甲烷作用
Environ Microbiol. 2004 Mar;6(3):274-87. doi: 10.1111/j.1462-2920.2004.00568.x.
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Microbial communities associated with geological horizons in coastal subseafloor sediments from the sea of okhotsk.鄂霍次克海沿岸海底沉积物中与地质层相关的微生物群落。
Appl Environ Microbiol. 2003 Dec;69(12):7224-35. doi: 10.1128/AEM.69.12.7224-7235.2003.
4
Assessment of bacterial community structure in the deep sub-seafloor biosphere by 16S rDNA-based techniques: a cautionary tale.基于16S rDNA技术对深海海底生物圈细菌群落结构的评估：一个警示故事。
J Microbiol Methods. 2003 Oct;55(1):155-64. doi: 10.1016/s0167-7012(03)00140-4.
5
Molecular characterization of sulfate-reducing bacteria in the Guaymas Basin.瓜伊马斯盆地硫酸盐还原菌的分子特征
Appl Environ Microbiol. 2003 May;69(5):2765-72. doi: 10.1128/AEM.69.5.2765-2772.2003.
6
Cultivating the uncultured.培养未培养的微生物。
Proc Natl Acad Sci U S A. 2002 Nov 26;99(24):15681-6. doi: 10.1073/pnas.252630999. Epub 2002 Nov 18.
7
Identification of active methylotroph populations in an acidic forest soil by stable-isotope probing.通过稳定同位素探测鉴定酸性森林土壤中的活性甲基营养菌种群。
Microbiology (Reading). 2002 Aug;148(Pt 8):2331-2342. doi: 10.1099/00221287-148-8-2331.
8
Microbial communities from methane hydrate-bearing deep marine sediments in a forearc basin.弧前盆地中含甲烷水合物的深海沉积物中的微生物群落。
Appl Environ Microbiol. 2002 Aug;68(8):3759-70. doi: 10.1128/AEM.68.8.3759-3770.2002.
9
PRIMROSE: a computer program for generating and estimating the phylogenetic range of 16S rRNA oligonucleotide probes and primers in conjunction with the RDP-II database.报春花：一种与RDP-II数据库结合使用，用于生成和估计16S rRNA寡核苷酸探针及引物系统发育范围的计算机程序。
Nucleic Acids Res. 2002 Aug 1;30(15):3481-9. doi: 10.1093/nar/gkf450.
10
Enumeration of 16S rDNA of Desulfotomaculum lineage 1 in rice field soil by real-time PCR with SybrGreen detection.利用SybrGreen检测的实时PCR技术对稻田土壤中脱硫肠状菌属谱系1的16S rDNA进行计数。
J Microbiol Methods. 2002 Jul;50(2):155-64. doi: 10.1016/s0167-7012(02)00024-6.

最初在深海沉积物中发现的一种通过16S rRNA基因序列鉴定的新型细菌分类广泛存在。

Widespread occurrence of a novel division of bacteria identified by 16S rRNA gene sequences originally found in deep marine sediments.

作者信息

Webster Gordon, Parkes R John, Fry John C, Weightman Andrew J

机构信息

Cardiff School of Biosciences, University of Cardiff, Main Building, Park Pl., P.O. Box 915, Cardiff CF10 3TL, Wales, United Kingdom.

出版信息

Appl Environ Microbiol. 2004 Sep;70(9):5708-13. doi: 10.1128/AEM.70.9.5708-5713.2004.

DOI:10.1128/AEM.70.9.5708-5713.2004
PMID:15345467
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC520855/
Abstract

Phylogenetic analysis of 16S rRNA gene sequences from deep marine sediments identified a deeply branching clade, designated candidate division JS1. Primers for PCR amplification of partial 16S rRNA genes that target the JS1 division were developed and used to detect JS1 sequences in DNA extracted from various sedimentary environments, including, for the first time, coastal marine and brackish sediments.

摘要

对来自深海沉积物的16S rRNA基因序列进行系统发育分析，鉴定出一个深度分支的进化枝，命名为候选分类群JS1。开发了用于PCR扩增靶向JS1分类群的部分16S rRNA基因的引物，并用于检测从各种沉积环境中提取的DNA中的JS1序列，其中包括首次检测的沿海海洋和咸淡水沉积物中的JS1序列。