Bundesanstalt für Geowissenschaften und Rohstoffe, Hannover, Germany.
FEMS Microbiol Ecol. 2010 May;72(2):198-207. doi: 10.1111/j.1574-6941.2010.00838.x. Epub 2010 Jan 19.
Sequences of members of the bacterial candidate division JS-1 and the classes Anaerolineae and Caldilineae of the phylum Chloroflexi are frequently found in 16S rRNA gene clone libraries obtained from marine sediments. Using a newly designed quantitative, real-time PCR assay, these bacterial groups were jointly quantified in samples from near-surface and deeply buried marine sediments from the Peru margin, the Black Sea, and a forearc basin off the island of Sumatra. In near-surface sediments, sequences of the JS-1 as well as Anaerolineae- and Caldilineae-related Bacteria were quantified with significantly lower 16S rRNA gene copy numbers than the sequences of total Bacteria. In contrast, in deeply buried sediments below approximately 1 m depth, similar quantities of the 16S rRNA gene copies of these specific groups and Bacteria were found. This finding indicates that JS-1 and Anaerolineae- and Caldilineae-related Bacteria might dominate the bacterial community in deeply buried marine sediments and thus seem to play an important ecological role in the deep biosphere.
在海洋沉积物的 16S rRNA 基因克隆文库中，经常发现细菌候选门 JS-1 成员以及绿弯菌门的 Anaerolineae 和 Caldilineae 类的序列。使用新设计的定量实时 PCR 检测方法，共同定量了来自秘鲁大陆架、黑海和苏门答腊岛前缘盆地的近地表和深埋海洋沉积物样本中的这些细菌群。在近地表沉积物中，JS-1 以及与 Anaerolineae 和 Caldilineae 相关的细菌的序列的 16S rRNA 基因拷贝数明显低于总细菌的序列。相比之下，在深度约 1 米以下的深埋沉积物中，发现这些特定群组和细菌的 16S rRNA 基因拷贝数相似。这一发现表明，JS-1 以及与 Anaerolineae 和 Caldilineae 相关的细菌可能主导深埋海洋沉积物中的细菌群落，因此在深部生物圈中似乎发挥着重要的生态作用。
