Di Rocco R J, Rumsey W L, Kuczynski B L, Linder K E, Pirro J P, Narra R K, Nunn A D
Bristol-Myers Squibb Pharmaceutical Research Institute, New Brunswick, New Jersey.
J Nucl Med. 1992 Jun;33(6):1152-9.
We have compared apparent myocardial blood flow (MBFapparent) indicated by 99mTc-teboroxime, 96Tc-sestamibi and 201TI to true MBF indicated by radiolabeled microscopheres using a technique for the co-injection of four radionuclides in the same animal. Studies were performed using rats in a single-pass model to obtain global MBF and using dogs in a multiple-pass model to determine regional MBF. To provide a wide range of MBF, adenosine was administered intravenously and the left anterior descending coronary artery was then ligated in the dogs, or hypercapnia was induced by decreasing respiratory frequency in the rats. The microsphere formula for determining MBF was applied to all agents. When MBFapparent was plotted as a function of true MBF, the ability of each agent to measure changes in true MBF was demonstrated by the proximity of the plotted function to the line of identity. For both the single and multiple-pass studies, statistical analysis of the nonlinear relationship between MBFapparent and true MBF showed that 201TI and 99mTc-teboroxime approximate true MBF better than 96Tc-sestamibi (p less than 0.001) under the conditions used in the present studies. In the single-pass studies, 99mTc-teboroxime approximated true MBF better than 201Tl (p less than 0.05), but in the multiple-pass experiments, 201Tl approximated true MBF better than 99mTc-teboroxime in only one dog (p less than 0.01) with no difference in the other two. Determination of the permeability-surface area product, PS, for each agent shows that the higher fidelity to true MBF obtained with 201Tl and 99mTc-teboroxime is related to substantially greater PS values for these agents relative to 96Tc-sestamibi.
我们采用在同一动物体内共注射四种放射性核素的技术，比较了用锝-99m替硼肟、锝-96司他米比和铊-201所显示的表观心肌血流量（MBFapparent）与用放射性标记微球所显示的真实MBF。研究在大鼠的单通道模型中进行以获得整体MBF，在犬的多通道模型中进行以确定局部MBF。为了提供广泛的MBF范围，对犬静脉注射腺苷，然后结扎左前降支冠状动脉，或通过降低大鼠呼吸频率诱导高碳酸血症。用于确定MBF的微球公式适用于所有药物。当将MBFapparent绘制为真实MBF的函数时，通过绘制函数与恒等线的接近程度证明了每种药物测量真实MBF变化的能力。对于单通道和多通道研究，对MBFapparent与真实MBF之间的非线性关系进行统计分析表明，在本研究使用的条件下，铊-201和锝-99m替硼肟比锝-96司他米比更接近真实MBF（p小于0.001）。在单通道研究中，锝-99m替硼肟比铊-201更接近真实MBF（p小于0.05），但在多通道实验中，仅在一只犬中铊-201比锝-99m替硼肟更接近真实MBF（p小于0.01），另外两只犬无差异。对每种药物的通透表面积乘积PS的测定表明，铊-201和锝-99m替硼肟相对于锝-96司他米比具有更高的真实MBF保真度，这与这些药物的PS值显著更高有关。
