Costa M A, Fernandes M H
Instituto de Ciências Biomédicas Abel Salazar, Largo Abel Salazar, 4000 Porto, Portugal.
J Mater Sci Mater Med. 2000 Mar;11(3):141-53. doi: 10.1023/a:1008975507654.
Human osteoblastic alveolar bone cells were cultured for 28 days in control conditions and in the presence of three non-lethal concentrations of AISI 316L stainless steel (SS) corrosion products. Cells were exposed to SS corrosion products in two experimental situations: (i) in selected stages of the incubation time (during the first, second, third and fourth week of culture); and (ii) during the 28 days incubation period. Cultures were characterized for cell proliferation, total protein content, alkaline phosphatase activity (ALP) and ability to form mineralized deposits; culture media was analyzed for ionized calcium (Ca) and phosphorus (P) concentrations throughout the incubation period. The presence of SS corrosion products during the different stages of the incubation period did not significantly affect the cell proliferation; however, a significant dose-dependent deleterious effect was observed on the levels and pattern of ALP activity, concentration of ionized Ca and P in the culture medium and, also, ability to form mineralized deposits, especially in cultures exposed during the first and second week of culture (respectively, lag phase and exponential cell growth phase). Similar effects were observed in cultures exposed to the SS corrosion products during the 28 days incubation period. However, the presence of such products during the third week (when the mineralization process occurs) and, also, during the fourth week, resulted in little or no significant effects on the behavior of alveolar bone cells. Results suggested that SS corrosion products above certain non-lethal concentrations may disturb the proliferation/differentiation relationship of osteoblastic human alveolar bone cell cultures.
人成骨型牙槽骨细胞在对照条件下以及在三种非致死浓度的AISI 316L不锈钢（SS）腐蚀产物存在的情况下培养28天。细胞在两种实验情况下暴露于SS腐蚀产物：（i）在培养时间的选定阶段（培养的第一、第二、第三和第四周）；（ii）在28天的培养期内。对培养物进行细胞增殖、总蛋白含量、碱性磷酸酶活性（ALP）和形成矿化沉积物能力的表征；在整个培养期内分析培养基中的离子钙（Ca）和磷（P）浓度。在培养期的不同阶段存在SS腐蚀产物对细胞增殖没有显著影响；然而，观察到对ALP活性水平和模式、培养基中离子Ca和P的浓度以及形成矿化沉积物的能力有显著的剂量依赖性有害影响，特别是在培养的第一周和第二周（分别为延迟期和细胞指数生长期）暴露的培养物中。在28天培养期内暴露于SS腐蚀产物的培养物中也观察到类似的影响。然而，在第三周（矿化过程发生时）以及第四周存在此类产物，对牙槽骨细胞的行为几乎没有或没有显著影响。结果表明，高于某些非致死浓度的SS腐蚀产物可能会干扰人成骨型牙槽骨细胞培养物的增殖/分化关系。
