Sun Z L, Wataha J C, Hanks C T
Department of Biological Materials and Sciences, The University of Michigan School of Dentistry, Ann Arbor 48109-1078, USA.
J Biomed Mater Res. 1997 Jan;34(1):29-37. doi: 10.1002/(sici)1097-4636(199701)34:1<29::aid-jbm5>3.0.co;2-p.
The objective of this study was to evaluate the effects of metal ions, which may be released from orthopedic or dental implants, on osteoblast metabolism and differentiation. ROS 17/2.8 cells were cultured in F-12 medium for 7 days. Then Al+3, Co+2, Cr+3, Ni+2, Ti+4, and V+3 were added at concentrations less than their cytotoxic concentrations. After 3 days, DNA synthesis, succinate dehydrogenase activity, alkaline phosphatase (ALP) activity, and culture calcification were assessed. Northern blots were performed for ALP, osteocalcin (OCN), and osteopontin (OPN) mRNA transcription. The data indicated that Cr+3 and A1+3 had few inhibitory effects on ROS cell metabolism below their cytotoxic concentrations, Ni+2, Co+2, Ti+4, and V+3 affected all these parameters of ROS cell metabolism at concentrations below cytotoxic levels. For RNA analysis, A1+3 significantly suppressed the expression of ALP, OCN, and OPN at both cytotoxic and noncytoxic concentrations. Co+2 specifically suppressed ALP expression at cytotoxic concentrations. Cr+3 and Ni+2 inhibited OCN, OPN, and ALP gene expression only at cytotoxic concentrations. For Ti+4 and V+3 ions, gene expression at cytotoxic levels was not significantly affected as compared with the effects at noncytotoxic level. These results show that metal ions may alter osteoblast behavior even at subtoxic concentrations, but do not always affect the expression of all genes similarly.
本研究的目的是评估可能从骨科或牙科植入物中释放的金属离子对成骨细胞代谢和分化的影响。将ROS 17/2.8细胞在F - 12培养基中培养7天。然后以低于其细胞毒性浓度的浓度添加Al+3、Co+2、Cr+3、Ni+2、Ti+4和V+3。3天后，评估DNA合成、琥珀酸脱氢酶活性、碱性磷酸酶（ALP）活性和培养物钙化情况。对ALP、骨钙素（OCN）和骨桥蛋白（OPN）mRNA转录进行Northern印迹分析。数据表明，在低于其细胞毒性浓度时，Cr+3和A1+3对ROS细胞代谢几乎没有抑制作用，Ni+2、Co+2、Ti+4和V+3在低于细胞毒性水平的浓度下影响ROS细胞代谢的所有这些参数。对于RNA分析，A1+3在细胞毒性和非细胞毒性浓度下均显著抑制ALP、OCN和OPN的表达。Co+2在细胞毒性浓度下特异性抑制ALP表达。Cr+3和Ni+2仅在细胞毒性浓度下抑制OCN、OPN和ALP基因表达。对于Ti+4和V+3离子，与非细胞毒性水平的影响相比，细胞毒性水平下的基因表达没有受到显著影响。这些结果表明，金属离子即使在亚毒性浓度下也可能改变成骨细胞行为，但并不总是以相同方式影响所有基因的表达。
