七（6-氨基-6-脱氧）-β-环糊精作为一种手性选择剂用于通过毛细管电泳分离阴离子分析物对映体。

Heptakis(6-amino-6-deoxy)-beta-cyclodextrin as a chiral selector for the separation of anionic analyte enantiomers by capillary electrophoresis.

作者信息

Budanova Natalia, Shapovalova Elena, Lopatin Sergei, Varlamov Valery, Shpigun Oleg

机构信息

Analytical Chemistry Division, Chemistry Department, Moscow State University, Russia.

出版信息

Electrophoresis. 2004 Aug;25(16):2795-800. doi: 10.1002/elps.200405970.

DOI:10.1002/elps.200405970

PMID:15352011

Abstract

A hepta-substituted beta-cyclodextrin bearing seven amino groups, heptakis(6-amino-6-deoxy)-beta-cyclodextrin (per-6-NH2-beta-CD) was successfully used as a chiral selector for the enantioseparation of different anionic analytes. The running buffer pH and chiral selector concentration were the studied parameters crucial in achieving the maximum possible enantioresolution. Enantiomeric separation of a mixture of seven carboxybenzyl-amino acids was achieved in 24 min. Excellent resolution was obtained for carboxybenzyl-tryptophan (Rs = 11.2).

摘要

一种带有七个氨基的七取代β-环糊精，即七（6-氨基-6-脱氧）-β-环糊精（全-6-NH2-β-CD），成功用作手性选择剂，用于不同阴离子分析物的对映体分离。运行缓冲液pH值和手性选择剂浓度是实现最大可能对映体拆分的关键研究参数。在24分钟内实现了七种羧苄基氨基酸混合物的对映体分离。羧苄基色氨酸获得了出色的分离度（Rs = 11.2）。