Tang Weihua, Muderawan I Wayan, Ng Siu Choon, Chan Hardy Sze On
Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore.
J Chromatogr A. 2005 Nov 11;1094(1-2):187-91. doi: 10.1016/j.chroma.2005.07.082. Epub 2005 Oct 17.
A facile synthetic approach for mono-6-amino-6-deoxy-beta-cyclodextrin (beta-CD-NH2) was proposed. Its hydroxy chloride salt, mono-6-ammonium-6-deoxy-beta-cyclodextrin chloride (beta-CD-NH3Cl) was further prepared and used for the enantioseparation of various anionic and ampholytic analytes by capillary electrophoresis (CE). The effect of background electrolyte (BGE) pH and selector concentration on the enantioseparation was studied. Results showed that beta-CD-NH3Cl displayed powerful chiral resolution ability towards anionic analytes. In addition, baseline separation of a standard mixture consisting of eight acids was achieved within 35 min.
提出了一种简便的合成单-6-氨基-6-脱氧-β-环糊精（β-CD-NH2）的方法。进一步制备了其羟基氯盐，单-6-铵基-6-脱氧-β-环糊精氯化物（β-CD-NH3Cl），并将其用于通过毛细管电泳（CE）对各种阴离子和两性分析物进行对映体分离。研究了背景电解质（BGE）pH值和选择剂浓度对对映体分离的影响。结果表明，β-CD-NH3Cl对阴离子分析物具有强大的手性拆分能力。此外，在35分钟内实现了由八种酸组成的标准混合物的基线分离。
