单-6-氯化铵-6-脱氧-β-环糊精作为毛细管电泳手性选择剂的合成与应用

Synthesis and application of mono-6-ammonium-6-deoxy-beta-cyclodextrin chloride as chiral selector for capillary electrophoresis.

作者信息

Tang Weihua, Muderawan I Wayan, Ng Siu Choon, Chan Hardy Sze On

机构信息

Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore.

出版信息

J Chromatogr A. 2005 Nov 11;1094(1-2):187-91. doi: 10.1016/j.chroma.2005.07.082. Epub 2005 Oct 17.

DOI:10.1016/j.chroma.2005.07.082

PMID:16229852

Abstract

A facile synthetic approach for mono-6-amino-6-deoxy-beta-cyclodextrin (beta-CD-NH2) was proposed. Its hydroxy chloride salt, mono-6-ammonium-6-deoxy-beta-cyclodextrin chloride (beta-CD-NH3Cl) was further prepared and used for the enantioseparation of various anionic and ampholytic analytes by capillary electrophoresis (CE). The effect of background electrolyte (BGE) pH and selector concentration on the enantioseparation was studied. Results showed that beta-CD-NH3Cl displayed powerful chiral resolution ability towards anionic analytes. In addition, baseline separation of a standard mixture consisting of eight acids was achieved within 35 min.

摘要

提出了一种简便的合成单-6-氨基-6-脱氧-β-环糊精（β-CD-NH2）的方法。进一步制备了其羟基氯盐，单-6-铵基-6-脱氧-β-环糊精氯化物（β-CD-NH3Cl），并将其用于通过毛细管电泳（CE）对各种阴离子和两性分析物进行对映体分离。研究了背景电解质（BGE）pH值和选择剂浓度对对映体分离的影响。结果表明，β-CD-NH3Cl对阴离子分析物具有强大的手性拆分能力。此外，在35分钟内实现了由八种酸组成的标准混合物的基线分离。