双色激发扫描显微镜中光学切片的改进

Optical-sectioning improvement in two-color excitation scanning microscopy.

作者信息

Ibáñez-López Cristina, Escobar Isabel, Saavedra Genaro, Martínez-Corral Manuel

机构信息

Department of Optics, University of Valencia, E46100 Burjassot, Spain.

出版信息

Microsc Res Tech. 2004 Jun 1;64(2):96-102. doi: 10.1002/jemt.20073.

Abstract

We present a new beam-shaping technique for two-color excitation fluorescence microscopy. We show that by simply inserting a properly designed shaded-ring filter in the illumination beam of smaller wavelength, it is possible to improve the effective optical sectioning capacity of such microscopes by 23%. Such an improvement is obtained at the expense of only a very small increasing of the overall energy in the point-spread-function sidelobes. The performance of this technique is illustrated by a numerical imaging simulation.

摘要

我们提出了一种用于双色激发荧光显微镜的新型光束整形技术。我们表明,通过在较小波长的照明光束中简单地插入一个设计合理的带阴影环滤波器,可以将此类显微镜的有效光学切片能力提高23%。这种改进仅以点扩散函数旁瓣中总能量的非常小的增加为代价获得。通过数值成像模拟说明了该技术的性能。

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