Valiullin V V, Deviataev A M, Islamov R R, Valiullina M E, Teplov A Iu
Biull Eksp Biol Med. 1992 Feb;113(2):198-200.
The immunohistochemical profile of intact and denervated soleus muscle of guinea pigs after sensibilization was studied. It is shown, that intact soleus muscle consists of slow fibers, which have low ATP-ase activity and don't react with monoclonal antibodies against fast myosin heavy chain. No changes of immunohistochemical profile were found after denervation or sensibilization. At the same time, the fibers, reacting with monoclonal antibodies against fast myosin heavy chain and having low ATP-ase activity, were found in denervated muscles after sensibilization. It is concluded, that the synthesis of fast myosin is induced after sensibilization of denervated muscles. Validity of myosin ATP-ase histochemistry for muscle fibers typing is discussed.
研究了致敏后豚鼠完整和去神经比目鱼肌的免疫组织化学特征。结果表明，完整的比目鱼肌由慢肌纤维组成，其具有低ATP酶活性，且不与抗快肌肌球蛋白重链的单克隆抗体发生反应。去神经或致敏后未发现免疫组织化学特征有变化。同时，在致敏后的去神经肌肉中发现了与抗快肌肌球蛋白重链单克隆抗体发生反应且具有低ATP酶活性的纤维。得出的结论是，去神经肌肉致敏后诱导了快肌肌球蛋白的合成。讨论了肌球蛋白ATP酶组织化学在肌纤维分型中的有效性。
