D'Orsi C J, Reale F R, Davis M A, Brown V J
Department of Radiology, University of Massachusetts Medical Center, Worcester 01655.
Radiology. 1992 Mar;182(3):801-3. doi: 10.1148/radiology.182.3.1535898.
This study was initiated to determine whether the apparent calcium loss during histologic breast specimen processing could be explained by the presence of birefringent, transparent calcium oxalate crystals (type I). In previous investigations, the authors had noted a possible loss of 26.2% of calcium during the processing and sectioning of breast specimens. Two hundred thirteen histologic slides prepared from blocks demonstrating calcium radiographically but not histologically were reviewed with polarized light. An additional 506 slides from 19 malignancies appearing as microcalcifications were also reviewed with polarized light. Only one slide from each group (0.2% and 0.5% from the malignant and benign groups, respectively) demonstrated birefringent calcium oxalate (type I). Thus, the presence of calcium oxalate does not sufficiently explain the non-visualization of calcium, which is due instead to processing of breast specimens.
开展本研究是为了确定在乳腺组织标本处理过程中出现的明显钙流失现象，是否可由双折射透明草酸钙晶体（I型）的存在来解释。在之前的研究中，作者注意到在乳腺标本的处理和切片过程中，钙可能会流失26.2%。对213张从经放射学检查显示有钙但组织学检查未发现钙的组织块制备的组织切片，进行了偏振光检查。另外，对来自19例表现为微钙化的恶性肿瘤的506张切片，也进行了偏振光检查。每组仅各有一张切片（分别占恶性和良性组的0.2%和0.5%）显示出双折射草酸钙（I型）。因此，草酸钙的存在并不能充分解释钙无法显示的原因，钙无法显示反而归因于乳腺标本的处理过程。
