Kusudo Kazuhito, Ishii Kaori, Rahman Matlubur, Aki Yasuharu, Miyatake Akira, Kosaka Hiroaki, Kimura Shoji, Komatsu Tatsuhiko, Yokoyama Masataka, Morita Kiyoshi, Abe Youichi, Nishiyama Akira
Department of Anesthesiology, Mitoyo General Hospital, Kagawa 769-1695, Japan.
Eur J Pharmacol. 2004 Sep 13;498(1-3):267-73. doi: 10.1016/j.ejphar.2004.07.109.
We previously demonstrated that intrarenal nitric oxide (NO) levels and renal blood flow are reduced during halothane anesthesia. Studies were performed to determine if volatile anesthetics-induced reductions in renal NO levels are associated with blood flow changes. Halothane and sevoflurane at 0.8 and 2.4 Mac were administered by inhalation to dogs, and cGMP and NOx concentrations in the renal interstitial fluid were measured by a microdialysis method. Neither halothane nor sevoflurane at 0.8 Mac altered renal blood flow and renal interstitial cyclic guanosine monophosphate (cGMP) and NOx levels, but both anesthetics significantly decreased these values at 2.4 Mac. Using an adjustable aortic clamp, renal perfusion pressure was reduced in 2 steps without halothane and sevoflurane anesthesia. Renal blood flow as well as cGMP and NOx concentrations in the renal interstitial fluid were unchanged within the autoregulatory range, but significantly decreased below the autoregulatory range. Changes in cGMP and NOx concentrations in the renal interstitial fluid were highly correlated with renal blood flow changes during halothane or sevoflurane anesthesia, and during stepwise reductions in renal perfusion pressure. The results suggested that halothane- and sevoflurane-induced decreases in intrarenal NO levels result from reductions in blood flow.
我们之前证明，在氟烷麻醉期间肾内一氧化氮（NO）水平和肾血流量会降低。开展研究以确定挥发性麻醉剂引起的肾NO水平降低是否与血流变化有关。通过吸入向犬给予0.8和2.4 MAC的氟烷和七氟烷，并采用微透析法测量肾间质液中的环磷酸鸟苷（cGMP）和NOx浓度。0.8 MAC的氟烷和七氟烷均未改变肾血流量以及肾间质环磷酸鸟苷（cGMP）和NOx水平，但两种麻醉剂在2.4 MAC时均显著降低了这些值。使用可调节的主动脉夹，在无氟烷和七氟烷麻醉的情况下分两步降低肾灌注压。在自动调节范围内，肾血流量以及肾间质液中的cGMP和NOx浓度未发生变化，但在低于自动调节范围时显著降低。在氟烷或七氟烷麻醉期间以及肾灌注压逐步降低期间，肾间质液中cGMP和NOx浓度的变化与肾血流量变化高度相关。结果表明氟烷和七氟烷引起的肾内NO水平降低是由血流减少所致。
