木聚糖酶在木浆漂白过程中对木质纤维素成分的影响。
The effect of xylanase on lignocellulosic components during the bleaching of wood pulps.
作者信息
Roncero M Blanca, Torres Antonio L, Colom José F, Vidal Teresa
机构信息
Department of Textile and Paper Engineering, E.T.S.E.I.T. Polytechnical University of Catalonia. Colom 11, 08222 Terrassa, Spain.
出版信息
Bioresour Technol. 2005 Jan;96(1):21-30. doi: 10.1016/j.biortech.2004.03.003.
HPLC, SEM and XRD techniques have been proposed as methods for ascertaining the changes occurring in polysaccharides (cellulose and xylans) and fibres during the xylanase bleaching processes. TCF and ECF bleached pulps with and without enzyme pretreatment were analysed. The ratio of carbohydrates present in the pulp, observation of changes occurring in the surface of the fibres and the crystallinity and accessibility of the bleached fibres were determinated. These characteristics have been related with pulp properties. Xylan content decreased when pulp was bleached. Xylanase treatment substantially reduced the xylose content present in pulp, measured by HPLC after the hydrolysis method of the sample. Morphological changes in the fibres occurred when the enzymatic treatment was applied. Bleaching increased the crystallinity of the pulp and enzyme pretreatment also affected the crystallinity of cellulose fibres
高效液相色谱法（HPLC）、扫描电子显微镜（SEM）和X射线衍射（XRD）技术已被提议作为确定木聚糖酶漂白过程中多糖（纤维素和木聚糖）和纤维发生变化的方法。对经过和未经过酶预处理的全无氯（TCF）和含氯无元素（ECF）漂白浆进行了分析。测定了纸浆中碳水化合物的比例、纤维表面发生的变化以及漂白纤维的结晶度和可及性。这些特性与纸浆性能相关。纸浆漂白时木聚糖含量降低。通过对样品进行水解法处理后用HPLC测定，木聚糖酶处理大大降低了纸浆中木糖的含量。进行酶处理时，纤维会发生形态变化。漂白提高了纸浆的结晶度，酶预处理也会影响纤维素纤维的结晶度。
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