Kainuma Kazuyuki, Katsuno Satoshi, Hashimoto Shigenari, Suzuki Nobuyoshi, Oguchi Tomohiro, Asamura Kenji, Nakajima Kosei, Usami Shin-ichi
Department of Otorhinolaryngology, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto 390-8621, Japan.
Auris Nasus Larynx. 2004 Sep;31(3):261-8. doi: 10.1016/j.anl.2004.03.012.
The final goal of this study is to develop a pre-operative fine needle aspiration biopsy (FNA) diagnostic system based on gene expression profiles. As the first step to that end, the present study was performed to determine whether the cDNA microarray system is applicable for histological evaluation of parotid gland tumors.
We investigated molecular characteristics on the basis of gene expression patterns of the two most common types of salivary gland tumors (pleomorphic adenomas and Warthin tumors) and normal salivary gland tissues, using the cDNA microarray system.
Pleomorphic adenomas and Warthin tumors can be classified by cDNA microarray. In pleomorphic adenomas, 11 independent genes were found to be up-regulated and 2 genes were down-regulated. In Warthin tumors, five independent genes were found to be up-regulated, and six genes were down-regulated. In hierarchical clustering analysis, cases were further grouped into two clusters according to the histological type. Furthermore, cDNA microarray enabled pleomorphic adenomas to be subclassified into three clusters according to the histological subtypes.
This study suggested that cDNA microarray may be useful and applicable for the pre-operative diagnosis (such as FNA) of the salivary gland tumor.
本研究的最终目标是开发一种基于基因表达谱的术前细针穿刺活检（FNA）诊断系统。作为实现该目标的第一步，本研究旨在确定cDNA微阵列系统是否适用于腮腺肿瘤的组织学评估。
我们使用cDNA微阵列系统，基于两种最常见的唾液腺肿瘤（多形性腺瘤和沃辛瘤）以及正常唾液腺组织的基因表达模式，研究其分子特征。
多形性腺瘤和沃辛瘤可以通过cDNA微阵列进行分类。在多形性腺瘤中，发现11个独立基因上调，2个基因下调。在沃辛瘤中，发现5个独立基因上调，6个基因下调。在层次聚类分析中，病例根据组织学类型进一步分为两个簇。此外，cDNA微阵列能够根据组织学亚型将多形性腺瘤再细分为三个簇。
本研究表明，cDNA微阵列可能对唾液腺肿瘤的术前诊断（如FNA）有用且适用。
