Gagliardi Maria Cristina, Teloni Raffaela, Mariotti Sabrina, Iona Elisabetta, Pardini Manuela, Fattorini Lanfranco, Orefici Graziella, Nisini Roberto
Dipartimento di Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanità, 00161 Rome, Italy.
Vaccine. 2004 Sep 28;22(29-30):3848-57. doi: 10.1016/j.vaccine.2004.07.009.
The only available vaccine against tuberculosis (TB) is Bacillus Calmette-Guérin (BCG) whose efficacy in preventing pulmonary tuberculosis is however controversial. Here, we show that BCG infection of monocytes causes their differentiation into mature dendritic cells (DCs) lacking CD1 molecules expression, coupled with suboptimal up-regulation of HLA class II, CD80 and CD40 molecules and a marked unresponsiveness to lipopolysaccharide stimulation. In addition, alloreactive naïve T lymphocytes primed by these subverted DCs did not undergo defined functional polarization, as witnessed by their inability to produce IFN-gamma. Since efficient antigen presentation and IFN-gamma production by mycobacterial-specific T lymphocytes are required for protection against Mycobacterium tuberculosis, our data might provide additional explanation for the low efficacy of BCG vaccination.
唯一可用于预防结核病（TB）的疫苗是卡介苗（BCG），但其预防肺结核的效果存在争议。在此，我们发现单核细胞受卡介苗感染后会分化为缺乏CD1分子表达的成熟树突状细胞（DCs），同时HLA II类分子、CD80和CD40分子的上调程度欠佳，并且对脂多糖刺激表现出明显的无反应性。此外，由这些被颠覆的树突状细胞激活的同种异体初始T淋巴细胞未发生明确的功能极化，这表现为它们无法产生γ干扰素。由于针对结核分枝杆菌的保护需要分枝杆菌特异性T淋巴细胞进行有效的抗原呈递和产生γ干扰素，我们的数据可能为卡介苗接种效果不佳提供了额外的解释。
