Jathoul Amit P, Holley Jane L, Garmory Helen S
Department of Biomedical Sciences, Defence Science and Technology Laboratory, Porton Down, Salisbury SP4 0JQ, UK.
Vaccine. 2004 Sep 28;22(29-30):3942-6. doi: 10.1016/j.vaccine.2004.04.006.
DNA vaccines which expressed the Hc fragment of the Clostridium botulinum type F neurotoxin (BoNT/F Hc) fused to a signal peptide downstream of four different eukaryotic promoters were prepared. Subsequently, the immunogenicity of the DNA vaccines and protection afforded in mice against challenge with 10(4) MLD of type F botulinum toxin was evaluated. The DNA vaccine containing the human ubiquitin gene (UbC) promoter induced the highest BoNT/F Hc-specific antibody concentration following two intramuscular immunisations and afforded 90% protection against challenge. The results from this study indicate that the selection of promoter used in DNA vaccination studies may be of importance in designing optimised vaccines.
制备了DNA疫苗，这些疫苗表达了与四种不同真核启动子下游的信号肽融合的F型肉毒杆菌神经毒素（BoNT/F Hc）的Hc片段。随后，评估了DNA疫苗的免疫原性以及在小鼠中针对10(4) MLD F型肉毒杆菌毒素攻击所提供的保护作用。含有人类泛素基因（UbC）启动子的DNA疫苗在两次肌肉内免疫后诱导出最高的BoNT/F Hc特异性抗体浓度，并提供了90%的攻击保护率。这项研究的结果表明，在DNA疫苗研究中使用的启动子选择对于设计优化疫苗可能很重要。
