Gardiner David F, Rosenberg Talia, Zaharatos Jerry, Franco David, Ho David D
Division of International Medicine and Infectious Diseases, Weill Cornell Medical College, New York, NY, United States.
Vaccine. 2009 Jun 2;27(27):3598-604. doi: 10.1016/j.vaccine.2009.03.058. Epub 2009 Apr 9.
Clostridium difficile is a pathogen with increasing severity for which host antibody responses provide protection from disease. DNA vaccination has several advantages compared to traditional vaccine methods, however no study has examined this platform against C. difficile toxins. A synthetic gene was created encoding the receptor-binding domain (RBD) of C. difficile toxin A, optimized for expression in human cells. Gene expression was examined in vitro. Mice were inoculated and then challenged with parenteral toxin A. Vaccination provided high titer antibodies and protected mice from death. This represents the first report of DNA vaccine inducing neutralizing antibodies to C. difficile toxin A.
艰难梭菌是一种致病性日益严重的病原体,宿主抗体反应可提供疾病防护。与传统疫苗方法相比,DNA疫苗接种有几个优点,然而尚无研究针对艰难梭菌毒素检测该平台。构建了一个合成基因,编码艰难梭菌毒素A的受体结合结构域(RBD),并针对在人细胞中的表达进行了优化。在体外检测了基因表达。给小鼠接种疫苗,然后用肠外毒素A进行攻击。疫苗接种产生了高滴度抗体并保护小鼠免于死亡。这是DNA疫苗诱导针对艰难梭菌毒素A的中和抗体的首次报道。