Yan Ye, Bian Wei, Xie Zhiqin, Cao Xinmin, Le Roux Isabelle, Guillemot Francois, Jing Naihe
Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
Dev Dyn. 2004 Oct;231(2):248-57. doi: 10.1002/dvdy.20126.
Stat3, a member of the signal transducer and activator of transcription (STAT) family, plays a central role in mediating cell growth, differentiation, and survival signals. In this report, we show that Stat3 immunoreactivity was localized to specific regions in the developing mouse brain, neural tube, and eye from embryonic day 10.5 to postnatal day 0. The active form of Stat3 protein, which is phosphorylated on tyrosine 705 (pYStat3), was also found in the developing neural tube with more restricted distribution. An in ovo chick embryo electroporation assay showed that the endogenous chick Stat3 could drive consensus sis-inducible element-directed reporter gene expression. These results demonstrate that the active Stat3 protein is present and might play a role during the development of the central nervous system and eye.
Stat3是信号转导子和转录激活子（STAT）家族的成员之一，在介导细胞生长、分化和存活信号方面发挥着核心作用。在本报告中，我们发现从胚胎第10.5天到出生后第0天，Stat3免疫反应性定位于发育中的小鼠脑、神经管和眼睛的特定区域。在发育中的神经管中也发现了在酪氨酸705位点磷酸化的Stat3蛋白的活性形式（pYStat3），但其分布更局限。卵内鸡胚电穿孔试验表明，内源性鸡Stat3可驱动共有sis诱导元件导向的报告基因表达。这些结果表明，活性Stat3蛋白存在，并且可能在中枢神经系统和眼睛的发育过程中发挥作用。
