Duncan S A, Zhong Z, Wen Z, Darnell J E
Department of Molecular Cell Biology, Rockefeller University, New York, New York 10021, USA.
Dev Dyn. 1997 Feb;208(2):190-8. doi: 10.1002/(SICI)1097-0177(199702)208:2<190::AID-AJA6>3.0.CO;2-D.
Cytokine activation of gene expression can be mediated through signal transducer and activator of transcription (STAT) signaling pathways resulting in expression of target genes. Because many cytokines have important regulatory roles during early development, we wanted to ascertain whether STAT signaling was also active at this time and could therefore have important roles in mediating developmental processes. We have found that Stat1 and Stat3 mRNAs are present in both maternal and extraembryonic tissues during early postimplantation stages of murine development. Furthermore, analyses of STAT activity in E4.5-E9.5 decidual swellings by electrophoretic mobility shift assay demonstrated that Stat3 protein was active during this early developmental period. The identification of activated Stat3 demonstrates that STAT signaling functions during early postimplantation development in the mouse are likely to be important during early embryogenesis.
细胞因子对基因表达的激活可通过信号转导及转录激活因子（STAT）信号通路介导，从而导致靶基因的表达。由于许多细胞因子在早期发育过程中具有重要的调节作用，我们想确定STAT信号在这个时期是否也具有活性，因此在介导发育过程中可能发挥重要作用。我们发现，在小鼠发育的植入后早期阶段，Stat1和Stat3 mRNA存在于母体和胚外组织中。此外，通过电泳迁移率变动分析对E4.5 - E9.5蜕膜肿胀中的STAT活性进行分析表明，Stat3蛋白在这个早期发育阶段具有活性。活化的Stat3的鉴定表明，STAT信号在小鼠植入后早期发育过程中的功能在早期胚胎发生过程中可能很重要。
