Haponsaph Ronnarit, Darden Janice M, Chantakulkij Somsak, Sannoi Nongluck, de Souza Mark S, Brown Arthur E, Birx Deborah L, Polonis Victoria R, Pattanapanyasat Kovit
Center of Excellence for Flow Cytometry, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
Asian Pac J Allergy Immunol. 2004 Mar;22(1):39-48.
To determine whether CD8+ T lymphocytes from Thai donor cells are susceptible to HIV-1 infection, undepleted peripheral blood mononuclear cells (PBMC) and CD8-enriched PBMC were infected with HIV-1 Thai subtype B and CRF01_AE (E) primary isolates. Virus kinetics in HIV-1 infection of CD4+ and CD8+ T lymphocytes peaked at day 7 or 10 post infection (pi); the TCID50 used for cell infection was proportional to the level of p24 production in the cultures. We also found that the level of p24 antigen in the supernatants of infected undepleted PBMC was significantly higher than that of infected CD8-enriched PBMC. Interestingly, both single positive T lymphocytes (CD4+ and CD8+ T lymphocytes) as well as double positive CD4+/CD8+ T lymphocytes were infected with HIV-1. The double positive T lymphocytes in PBMC were found only in the presence of both CD4+ and CD8+ T lymphocytes. The majority of p24+/CD4-/CD8- T lymphocytes were HIV-1 infected CD4 down-modulated PBMC. This report provides direct evidence that single positive CD8+ T lymphocytes and double positive CD4+/ CD8+ T lymphocytes from Thai donors can be infected with HIV-1 subtypes B and E in vitro.
为了确定来自泰国供体细胞的CD8 + T淋巴细胞是否易受HIV-1感染，未耗尽的外周血单核细胞（PBMC）和富含CD8的PBMC用HIV-1泰国B亚型和CRF01_AE（E）原始分离株进行感染。CD4 +和CD8 + T淋巴细胞感染HIV-1后的病毒动力学在感染后（pi）第7天或第10天达到峰值；用于细胞感染的半数组织培养感染剂量（TCID50）与培养物中p24的产生水平成正比。我们还发现，未耗尽的受感染PBMC上清液中的p24抗原水平明显高于富含CD8的受感染PBMC。有趣的是，单阳性T淋巴细胞（CD4 +和CD8 + T淋巴细胞）以及双阳性CD4 + / CD8 + T淋巴细胞均感染了HIV-1。PBMC中的双阳性T淋巴细胞仅在同时存在CD4 +和CD8 + T淋巴细胞的情况下才被发现。大多数p24 + / CD4- / CD8- T淋巴细胞是HIV-1感染的CD4下调的PBMC。本报告提供了直接证据，表明来自泰国供体的单阳性CD8 + T淋巴细胞和双阳性CD4 + / CD8 + T淋巴细胞在体外可被HIV-1 B亚型和E亚型感染。
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