Yamamura Y, Rodriguez N, Schwartz A, Eylar E, Yano N
Ponce School of Medicine, AIDS Research Program, Puerto Rico 00732.
Cell Mol Biol (Noisy-le-grand). 1995;41 Suppl 1:S133-44.
A new cell proliferation analysis by flow cytometry was applied to the mitogen induced cultures of peripheral blood mononuclear cells (PBMC) from either normal, healthy donors or those individuals who are infected by human immunodeficiency virus, type 1 (HIV-1). While phytohemagglutinin (PHA) stimulation of normal PBMC (nPBMC) yielded propagation of both CD4 (nCD4) and CD8 (nCD8) T cell subsets, similar activation of PBMC from certain HIV-1-infected individuals (HIV-PBMC) produced active proliferation of CD8 (HIV-CD8) cells but varying degrees of CD4 (HIV-CD4) cell destruction. However, no measurable viral p24 antigen was produced extracellularly. On the other hand, when the purified HIV-CD4 cells were similarly activated, no such cell death was noted and high titer p24 was detected in the culture supernatants. Addition of exogenous IL-2 to either HIV-PBMC or HIV-CD4 cultures, did not alter either CD4 cell death or HIV-1 p24 production. Isolated HIV-CD8 killed not only HIV-CD4 but also nCD4, when co-cultured and less proliferative fractions of CD4 cell population was the more preferred targets of the HIV-CD8 CTL activity. The presence of anti-CD4 CTL activity was closely associated with high CD8, but not with CD4 counts, of the HIV+ patients.
一种新的通过流式细胞术进行的细胞增殖分析应用于来自正常健康供体或感染1型人类免疫缺陷病毒（HIV-1）的个体的外周血单个核细胞（PBMC）的丝裂原诱导培养物。虽然植物血凝素（PHA）刺激正常PBMC（nPBMC）会导致CD4（nCD4）和CD8（nCD8）T细胞亚群的增殖，但对某些HIV-1感染个体（HIV-PBMC）的PBMC进行类似激活时，会使CD8（HIV-CD8）细胞活跃增殖，但CD4（HIV-CD4）细胞会受到不同程度的破坏。然而，细胞外未产生可测量的病毒p24抗原。另一方面，当纯化的HIV-CD4细胞被类似激活时，未观察到此类细胞死亡，且在培养上清液中检测到高滴度的p24。向HIV-PBMC或HIV-CD4培养物中添加外源性IL-2，既未改变CD4细胞死亡情况，也未改变HIV-1 p24的产生。当共培养时，分离出的HIV-CD8不仅杀死HIV-CD4，还杀死nCD4，且CD4细胞群体中增殖较少的部分是HIV-CD8细胞毒性T淋巴细胞（CTL）活性更倾向的靶标。抗CD4 CTL活性的存在与HIV+患者的高CD8密切相关，但与CD4计数无关。
