Liu Zhongmin, Xu Zhenping, Shen Wenlu, Li Yi, Zhang Jun, Ye Xin
Department of General Surgery and Organ Transplantation, the Second Affiliated Hospital, Shantou University Medical College, Dongxiabeilu, Shantou, 515041 Guangdong, People's Republic of China.
World J Surg. 2004 Jun;28(6):620-4. doi: 10.1007/s00268-004-7172-3.
The effect of tetrandrine (TET) pretreatment of Wistar rats subjected to warm hepatic ischemia/reperfusion (I/R) was investigated. After 50 minutes of ischemia in the left and median lobes of the liver and 24 hours of reperfusion (I/R group), the rats were killed. The TET+I/R group rats were pretreated with TET (50 mg/kg body weight IP) 30 minutes prior to the onset of ischemia. Blood samples were taken for measurement of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH). Tissue was taken from the ischemic lobes for measurement of superoxide dismutase (SOD), malonyldialdehyde (MDA), and myeloperoxidase (MPO); determination of the wet/dry weight (W/D) ratio; and histologic studies. The results showed that ALT, AST, and LDH levels in serum were increased in the I/R group; tissue MDA generation, MPO activity, and the W/D ratio were also increased, accompanied by decreased SOD activity. The serum ALT, AST, and LDH levels, as well as the tissue MPO level and W/D ratio, were lower in the TET+ I/R group than in the I/R group; and the SOD level was higher in the TET+IR group than in the I/R group. Moreover, the serum ALT and AST, tissue MDA, and W/D ratio in the TET+I/R group were higher, and the SOD was lower than in the sham group. The histologic examination showed protection against liver damage in the TET+I/R group. The results demonstrated that pretreatment with TET could somewhat protect the liver against I/R injury but does not prevent it. The simultaneous decrease of both lipid peroxide generation and polymorphonuclear neutrophil infiltration in the ischemic liver may explain the acquisition of tolerance following administration of TET.
研究了汉防己甲素（TET）预处理对经历肝脏热缺血/再灌注（I/R）的Wistar大鼠的影响。在左叶和中叶肝脏缺血50分钟并再灌注24小时后（I/R组），处死大鼠。TET+I/R组大鼠在缺血开始前30分钟用TET（50mg/kg体重，腹腔注射）进行预处理。采集血样以测定丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）和乳酸脱氢酶（LDH）。从缺血叶取材以测定超氧化物歧化酶（SOD）、丙二醛（MDA）和髓过氧化物酶（MPO）；测定湿/干重（W/D）比值；并进行组织学研究。结果显示，I/R组血清中ALT、AST和LDH水平升高；组织MDA生成、MPO活性和W/D比值也升高，同时SOD活性降低。TET+I/R组血清ALT、AST和LDH水平以及组织MPO水平和W/D比值低于I/R组；TET+I/R组SOD水平高于I/R组。此外，TET+I/R组血清ALT和AST、组织MDA和W/D比值高于假手术组，SOD低于假手术组。组织学检查显示TET+I/R组对肝损伤有保护作用。结果表明，TET预处理可在一定程度上保护肝脏免受I/R损伤，但不能完全预防。缺血肝脏中脂质过氧化物生成和多形核中性粒细胞浸润同时减少可能解释了给予TET后获得耐受性的原因。
