Sagiroglu Tamer, Aksoy Mustafa Burak, Sagiroglu Gonul, Tozkir Hilmi, Oguz Serhat, Yalta Tulin, Yagci Mehmet A, Sezer Atakan
Faculty of Medicine Department of General Surgery, Trakya University, Trakya Üniversitesi Tıp Fakültesi Genel Cerrahi, AD 22030 Edirne, Turkey.
Faculty of Medicine Department of Emergency Service, Trakya University, Trakya Üniversitesi Tıp Fakültesi Acil Servis, AD 22030 Edirne, Turkey.
Indian J Surg. 2014 Apr;76(2):111-6. doi: 10.1007/s12262-012-0676-4. Epub 2012 Jul 4.
Leptin and apelin are important adipocytokines involved in a variety of endocrine and paracrine functions. The aim of this study was to evaluate the effect of exogenous leptin and apelin preconditioning on hepatic ischemia reperfusion (I/R) injury in rats. Forty mice were assigned to four groups (n = 10): sham-operated control (sham), I/R injury, I/R + leptin (I/R + L), and I/R + apelin (I/R + A). Leptin 100 μg/kg/day and apelin 2 μg/kg/day were delivered intraperitoneally starting 3 days prior to surgical procedure in I/R + L and I/R + A groups, respectively. All I/R groups underwent 45 min of warm ischemia, followed by 30 min of reperfusion. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST), liver malondialdehyde (MDA) and glutathione (GSH), and liver histopathology were compared between groups. MDA was elevated in I/R, but stayed similar in I/R + L and I/R + A compared to sham. I/R + A had significantly lower MDA compared to I/R. GSH levels did not differ significantly between the groups. ALT and AST were elevated in all I/R groups, but significant reduction was observed in I/R + L and I/R + A compared to I/R. Liver histopathology was mostly mild in I/R + L and I/R + A, in contrast to severe injury observed in the I/R group. Leptin and apelin preconditioning significantly reduced hepatic I/R injury in rats.
瘦素和脂联素是参与多种内分泌和旁分泌功能的重要脂肪细胞因子。本研究的目的是评估外源性瘦素和脂联素预处理对大鼠肝脏缺血再灌注（I/R）损伤的影响。将40只小鼠分为四组（n = 10）：假手术对照组（假手术组）、I/R损伤组、I/R + 瘦素组（I/R + L）和I/R + 脂联素组（I/R + A）。在手术前3天，分别对I/R + L组和I/R + A组小鼠腹腔注射100 μg/kg/天的瘦素和2 μg/kg/天的脂联素。所有I/R组均经历45分钟的热缺血，随后进行30分钟的再灌注。比较各组血清丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）、肝脏丙二醛（MDA）和谷胱甘肽（GSH）水平以及肝脏组织病理学。I/R组MDA升高，但与假手术组相比，I/R + L组和I/R + A组MDA保持相似。与I/R组相比，I/R + A组MDA显著降低。各组GSH水平差异无统计学意义。所有I/R组ALT和AST均升高，但与I/R组相比，I/R + L组和I/R + A组显著降低。与I/R组观察到的严重损伤相反，I/R + L组和I/R + A组肝脏组织病理学大多为轻度。瘦素和脂联素预处理可显著减轻大鼠肝脏I/R损伤。
