利用傅里叶变换红外光谱和主成分分析鉴别完整和受损的单核细胞增生李斯特菌。
Discrimination of intact and injured Listeria monocytogenes by Fourier transform infrared spectroscopy and principal component analysis.
作者信息
Lin Mengshi, Al-Holy Murad, Al-Qadiri Hamzah, Kang Dong-Hyun, Cavinato Anna G, Huang Yiqun, Rasco Barbara A
机构信息
Department of Food Science and Human Nutrition, Box 646376, Washington State University, Pullman, Washington 99164-6373, USA.
出版信息
J Agric Food Chem. 2004 Sep 22;52(19):5769-72. doi: 10.1021/jf049354q.
Fourier transform infrared spectroscopy (FT-IR, 4000-600 cm(-)(1)) was used to discriminate between intact and sonication-injured Listeria monocytogenes ATCC 19114 and to distinguish this strain from other selected Listeria strains (L. innocua ATCC 51742, L. innocua ATCC 33090, and L. monocytogenes ATCC 7644). FT-IR vibrational overtone and combination bands from mid-IR active components of intact and injured bacterial cells produced distinctive "fingerprints" at wavenumbers between 1500 and 800 cm(-)(1). Spectral data were analyzed by principal component analysis. Clear segregations of different intact and injured strains of Listeria were observed, suggesting that FT-IR can detect biochemical differences between intact and injured bacterial cells. This technique may provide a tool for the rapid assessment of cell viability and thereby the control of foodborne pathogens.
傅里叶变换红外光谱法（FT-IR，4000 - 600 cm⁻¹）用于区分完整的和经超声处理损伤的单核细胞增生李斯特菌ATCC 19114，并将该菌株与其他选定的李斯特菌菌株（无害李斯特菌ATCC 51742、无害李斯特菌ATCC 33090和单核细胞增生李斯特菌ATCC 7644）区分开来。完整和受损细菌细胞的中红外活性成分的FT-IR振动泛音和组合带在1500至800 cm⁻¹的波数处产生独特的“指纹”。光谱数据通过主成分分析进行分析。观察到不同完整和受损李斯特菌菌株的明显分离，表明FT-IR可以检测完整和受损细菌细胞之间的生化差异。该技术可能为快速评估细胞活力从而控制食源性病原体提供一种工具。
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