Lasota B, Błaszczyk B, Seremak B, Udała J
Department of Animal Reproduction, Faculty of Biotechnology and Animal Husbandry, Agricultural University in Szczecin, Szczecin, Poland.
Reprod Domest Anim. 2004 Oct;39(5):309-14. doi: 10.1111/j.1439-0531.2004.00514.x.
This study was performed to determine the relationship between selenium (Se) content and Se-dependent glutathione peroxidase (GSH-Px) activity in blood and semen, and semen quality of boars at different age used in an artificial insemination (AI) station. Routine macroscopic and microscopic analyses of semen quality were accompanied by measurement of Se content and GSH-Px activity in blood and semen. The Se concentration in blood plasma, seminal fluid and spermatozoa was measured by fluorometric method, the GSH-Px activity by a method based on NADPH-coupled reaction. A total of 155 ejaculates and 58 blood samples were investigated. The results of this study showed that there was no direct relationship between the Se content and GSH-Px activity in blood plasma and semen, and semen quality of sexual matured boars. The mechanisms controlling Se content and GSH-Px activity in blood and semen seem to be independent. The age of boars as a differentiating factor for Se content and GSH-Px activity in blood and semen is possible. It is concluded that a determination of Se status and/or GSH-Px activity in organism before Se supplementation is indicated.
本研究旨在确定人工授精站不同年龄公猪血液和精液中硒（Se）含量与硒依赖性谷胱甘肽过氧化物酶（GSH-Px）活性之间的关系，以及精液质量。精液质量的常规宏观和微观分析同时测定血液和精液中的硒含量和GSH-Px活性。采用荧光法测定血浆、精液和精子中的硒浓度，采用基于NADPH偶联反应的方法测定GSH-Px活性。共调查了155份射精样本和58份血液样本。本研究结果表明，性成熟公猪血浆和精液中的硒含量与GSH-Px活性以及精液质量之间没有直接关系。控制血液和精液中硒含量和GSH-Px活性的机制似乎是独立的。公猪年龄可能是血液和精液中硒含量及GSH-Px活性的一个区分因素。得出结论，在补充硒之前，建议测定机体的硒状态状态和/或GSH-Px活性状态。
