Wang Y F, Yu M, te Pas M F W, Yerle M, Liu B, Fan B, Xiong T A, Li K
Lab of Molecular Biology and Animal Breeding, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China.
Anim Genet. 2004 Oct;35(5):361-6. doi: 10.1111/j.1365-2052.2004.01176.x.
The full-length cDNA of porcine genes (PSME1 and PSME2) encoding proteasome activators PA28alpha- and beta-subunits were obtained by the rapid amplification of cDNA ends (RACE). The nucleotide sequences and the predicted protein sequences share high sequence identity with their mammalian counterparts. The reverse transcriptase-polymerase chain reaction (RT-PCR) revealed that porcine PSME1 and PSME2 genes are expressed in all eight tissues studied (liver, spleen, bladder, small intestine, kidney, heart, skeletal muscle and lung). The full-length genomic DNA of the porcine PSME1 and PSME2 genes were amplified by PCR. These two genes shared the same structure and were similar in size. A C/T single nucleotide polymorphism in PSME1 intron 8 detected as an SphI PCR-restriction fragment length polymorphism (PCR-RFLP) shows allele frequency differences between Meishan, Tibetan, Large White, Qingping, and Duroc pigs. The association analysis using two experimental GY selection lines selected for growth rate or leanness suggested that the PSME1 genotype was associated with weaning weight. Analyses of somatic cell hybrid (SCHP) and radiation hybrid (IMpRH) panels revealed that both genes map to SSC7q15.3-q21 and closely linked to the T-cell receptor alpha (TCRA) gene.
通过cDNA末端快速扩增（RACE）获得了编码蛋白酶体激活剂PA28α和β亚基的猪基因（PSME1和PSME2）的全长cDNA。其核苷酸序列和预测的蛋白质序列与其哺乳动物对应物具有高度的序列同一性。逆转录聚合酶链反应（RT-PCR）显示，猪PSME1和PSME2基因在所研究的所有八个组织（肝脏、脾脏、膀胱、小肠、肾脏、心脏、骨骼肌和肺）中均有表达。通过PCR扩增了猪PSME1和PSME2基因的全长基因组DNA。这两个基因具有相同的结构且大小相似。在PSME1内含子8中检测到的一个C/T单核苷酸多态性，表现为SphI PCR-限制性片段长度多态性（PCR-RFLP），显示梅山猪、藏猪、大白猪、清平猪和杜洛克猪之间的等位基因频率存在差异。使用两个针对生长速度或瘦肉率进行选择的实验性GY选择系进行的关联分析表明，PSME1基因型与断奶体重相关。体细胞杂种（SCHP）和辐射杂种（IMpRH）板分析显示，这两个基因均定位于SSC7q15.3-q21，并且与T细胞受体α（TCRA）基因紧密连锁。
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