猪RPLP0基因全长cDNA的特征分析、染色体定位及多态性研究

Characterization of the full-length cDNA, chromosomal localization, and polymorphism of the porcine RPLP0 gene.

作者信息

Wu Xiao, Yang Shulin, Zhu Zhengmao, Feng Shutang, Li Kui

机构信息

Department of Gene and Cell Engineering, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China.

出版信息

J Genet Genomics. 2007 Feb;34(2):104-8. doi: 10.1016/S1673-8527(07)60011-3.

DOI:10.1016/S1673-8527(07)60011-3

PMID:17469782

Abstract

RPLP0 gene encodes the acidic ribosomal phosphoprotein large P0 subunit, which is a component of the 60S subunit. The full-length cDNA sequence of porcine RPLP0 was obtained from skeletal muscle of fetal pig cDNA library and deposited in GenBank. The nucleotide sequence and the predicted protein sequence shared high sequence identity with other mammalian homologues. A C/A single nucleotide substitution in exon 5 was detected as Csp6?polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) shows allele frequency diversity among Tongcheng, Xiaomeishan, Yushan, Large White, Landrace, and Duroc breeds. Analyses of somatic cell hybrid panel (SCHP) and radiation hybrid (IMpRH) panel showed that the RPLP0 gene was mapped to SSC 14q22-q24 and was closely linked to locus SW1321 (25 cR, LOD = 14.54).

摘要

RPLP0基因编码酸性核糖体磷蛋白大P0亚基，它是60S亚基的一个组成部分。猪RPLP0的全长cDNA序列从胎猪cDNA文库的骨骼肌中获得，并已存入GenBank。该核苷酸序列和预测的蛋白质序列与其他哺乳动物同源物具有高度的序列同一性。在外显子5中检测到一个C/A单核苷酸替换，因为Csp6?聚合酶链反应-限制性片段长度多态性（PCR-RFLP）显示了通城、小梅山、玉山、大白、长白和杜洛克品种之间的等位基因频率差异。体细胞杂种板（SCHP）和辐射杂种（IMpRH）板分析表明，RPLP0基因定位于猪14号染色体q22-q24区域，并且与SW1321位点紧密连锁（25 cR，LOD = 14.54）。