Dvash Tamar, Mayshar Yoav, Darr Henia, McElhaney Michael, Barker Douglas, Yanuka Ofra, Kotkow Karen J, Rubin Lee L, Benvenisty Nissim, Eiges Rachel
Department of Genetics, The Institute of Life Sciences, The Hebrew University, Jerusalem 91904, Israel.
Hum Reprod. 2004 Dec;19(12):2875-83. doi: 10.1093/humrep/deh529. Epub 2004 Sep 16.
The aim of this study was to characterize human embryonic stem (ES) cells at the molecular level by performing large-scale complementary DNA (cDNA) analysis using DNA micro-arrays.
The transcription profile of human ES cells was determined by comparing it to 2, 10 and 30-day old embryoid bodies (EBs) using Affymetrix Genechip human micro-arrays (U133).
According to this analysis we demonstrate that two human ES cell lines are more close to each other than to their differentiated derivatives. We also show the spectrum of cytokine receptors that they express, and demonstrate the presence of five genes that are highly specific to human ES cells and to germ cells. Moreover, by profiling different stages in the differentiation of human embryoid bodies, we illustrate the clustering of five sets of temporally expressed genes, which could be related to the sequential stages of embryonic development. Among them are known genes that are involved in early pattern formation.
The present study provides a molecular basis for the identity of human ES cells and demonstrates that during their in vitro differentiation they express embryonic specific genes in a stage specific manner.
本研究的目的是通过使用DNA微阵列进行大规模互补DNA（cDNA）分析，在分子水平上对人类胚胎干细胞（ES细胞）进行表征。
使用Affymetrix Genechip人类微阵列（U133），通过将人类ES细胞的转录谱与2、10和30天大的胚状体（EBs）进行比较来确定其转录谱。
根据该分析，我们证明两条人类ES细胞系彼此之间比它们分化后的衍生物更为相似。我们还展示了它们所表达的细胞因子受体谱，并证明存在五个对人类ES细胞和生殖细胞高度特异的基因。此外，通过分析人类胚状体分化的不同阶段，我们阐明了五组随时间表达的基因的聚类情况，这可能与胚胎发育的连续阶段有关。其中包括参与早期模式形成的已知基因。
本研究为人类ES细胞的特性提供了分子基础，并证明在其体外分化过程中，它们以阶段特异性方式表达胚胎特异性基因。
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