Xu Hongbin, Tanphaichitr Nongnuj, Forkert Poh-Gek, Anupriwan Araya, Weerachatyanukul Wattana, Vincent Renaud, Leader Arthur, Wade Michael G
Ottawa Health Research Institute, Hormones, Growth, Development, and Department of Biochemistry/Microbiology/Immunology, University of Ottawa, Ottawa, Ontario, Canada K1Y 4E9.
Toxicol Sci. 2004 Dec;82(2):590-7. doi: 10.1093/toxsci/kfh277. Epub 2004 Sep 16.
Trichloroethylene (TCE) is a prevalent occupational and environmental contaminant that has been reported to cause a variety of toxic effects. Here, we have undertaken studies to test the hypothesis that TCE exposure adversely affects sperm function and fertilization. Sperm retrieved from mice exposed to TCE (1000 ppm) by inhalation for 1 to 6 weeks were incubated in vitro with eggs isolated from superovulated female mice. The number of sperm bound per egg was significantly decreased when mice were exposed to TCE for 2 and 6 weeks but not at exposures of 1 and 4 weeks. In vivo fertilization was also determined in superovulated female mice mated with males exposed to TCE for 2 to 6 weeks. The percentages of eggs fertilized, as assessed by the presence of two pronuclei, were significantly decreased after 2 and 6 weeks of TCE exposure. A slight but insignificant decrease was observed after 4 weeks of TCE exposure. The direct effects of TCE and its metabolites, chloral hydrate (CH) and trichloroethanol (TCOH), on in vitro sperm-egg binding were also investigated. Sperm-egg binding was significantly decreased when sperm were pretreated with CH (0.1-10 microg/mL). Significantly lower levels of sperm-egg binding were also detected with TCOH (0.1-10 microg/mL), although the decreases were not as pronounced as those for CH. These results showed that TCE exposure leads to impairment of sperm fertilizing ability, which may be attributed to TCE metabolites, CH, and TCOH.
三氯乙烯（TCE）是一种常见的职业和环境污染物，据报道会导致多种毒性作用。在此，我们进行了研究以验证TCE暴露会对精子功能和受精产生不利影响这一假设。将通过吸入1至6周TCE（1000 ppm）的小鼠获取的精子与从超排卵雌性小鼠分离的卵子在体外进行孵育。当小鼠暴露于TCE 2周和6周时，每个卵子结合的精子数量显著减少，但在暴露1周和4周时未出现这种情况。还对与暴露于TCE 2至6周的雄性小鼠交配的超排卵雌性小鼠进行了体内受精测定。通过双原核的存在评估，TCE暴露2周和6周后受精卵子的百分比显著降低。TCE暴露4周后观察到轻微但不显著的下降。还研究了TCE及其代谢产物水合氯醛（CH）和三氯乙醇（TCOH）对体外精子 - 卵子结合的直接影响。当精子用CH（0.1 - 10微克/毫升）预处理时，精子 - 卵子结合显著减少。用TCOH（0.1 - 10微克/毫升）也检测到精子 - 卵子结合水平显著降低，尽管下降程度不如CH明显。这些结果表明，TCE暴露会导致精子受精能力受损，这可能归因于TCE代谢产物CH和TCOH。
