产甲烷菌中鞭毛蛋白糖基化与鞭毛丝对 Triton X-100 敏感性之间的相关性

Correlation between glycosylation of flagellin proteins and sensitivity of flagellar filaments to Triton X-100 in methanogens.

作者信息

Faguy D M, Koval S F, Jarrell K F

机构信息

Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada.

出版信息

FEMS Microbiol Lett. 1992 Jan 1;69(2):129-34. doi: 10.1016/0378-1097(92)90616-v.

DOI:10.1016/0378-1097(92)90616-v

PMID:1537540

Abstract

The flagellins of Methanospirillum hungatei strains JF1 and GP1, Methanococcus deltae, and Methanothermus fervidus are glycosylated. Isolated flagellar filaments from these organisms are dissociated by low concentrations (0.5% (v/v)) of Triton X-100. Flagellar filaments from other methanogens (Methanococcus voltae, Methanococcus vannielii and Methanoculleus marisnigri) composed of non-glycosylated flagellins are resistant to Triton X-100 treatment. Consequently, the isolation techniques (employing Triton X-100) used to isolate basal body-hook-filament complexes in eubacteria may not be applicable to many methanogens.

摘要

亨氏甲烷螺菌菌株JF1和GP1、三角洲甲烷球菌及嗜热甲烷栖热菌的鞭毛蛋白是糖基化的。从这些生物体中分离出的鞭毛丝可被低浓度（0.5%（v/v））的 Triton X-100解离。来自其他产甲烷菌（沃氏甲烷球菌、万氏甲烷球菌和黑海甲烷袋状菌）的由非糖基化鞭毛蛋白组成的鞭毛丝对Triton X-100处理具有抗性。因此，用于在真细菌中分离基体-钩-鞭毛丝复合物的分离技术（使用Triton X-100）可能不适用于许多产甲烷菌。