通过细胞融合和原生质体融合进行拟康氏木霉的种内杂交。
Intraspecific hybridisation of Trichoderma pseudokoningii by anastomosis and by protoplast fusion.
作者信息
Furlaneto M C, Pizzirani-Kleiner A A
机构信息
Department of Genetics, University of Sao Paulo, Brazil.
出版信息
FEMS Microbiol Lett. 1992 Jan 1;69(2):191-5. doi: 10.1016/0378-1097(92)90627-z.
Double auxotrophic and morphological mutants of Trichoderma pseudokoningii Rifai were fused by anastomosis and by protoplast fusion. The recovery of recombinants from heterokaryons on different selective media and from heterokaryotic colonies indicated the occurrence of parasexual events. Prototrophic colonies growing on minimal medium produced binucleate spores, green in colour, revealing a non-autonomous system for conidial pigmentation. Recombinants were obtained from these dikaryotic colonies suggesting the occurrence of a highly unstable diploid phase.
对拟康氏木霉（Trichoderma pseudokoningii Rifai）的双营养缺陷型和形态突变体进行了吻合和原生质体融合。在不同选择培养基上从异核体以及从异核菌落中获得重组体，这表明发生了准性生殖事件。在基本培养基上生长的原养型菌落产生双核孢子，颜色为绿色，揭示了分生孢子色素沉着的非自主系统。从这些双核菌落中获得了重组体，这表明存在高度不稳定的二倍体阶段。
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