Universidade Paranaense , Umuarama, PR , Brasil.
Braz J Microbiol. 2011 Oct;42(4):1625-37. doi: 10.1590/S1517-83822011000400050. Epub 2011 Dec 1.
With the aim of a better characterization of the somatic recombination process in Trichoderma pseudokoningii, a progeny from crossings between T. pseudokoningii strains contrasting for auxotroph markers was characterized by RAPD markers and PFGE (electrophoretic karyotype). Cytological studies of the conidia, conidiogenesis and heterokaryotic colonies were also performed. The genotypes of the majority of the recombinant strains analyzed were similar to only one of the parental strains and the low frequency of polymorphic RAPD bands suggested that the nuclear fusions may not occur into the heterokaryon. In some heterokaryotic regions the existence of intensely staining hyphae might be related to cell death. We proposed that a mechanism of somatic recombination other than parasexuality might occur, being related to limited vegetative compatibility after postfusion events, as described for other Trichoderma species.
为了更好地描述拟康氏木霉体细胞重组过程，利用 RAPD 标记和 PFGE（电泳核型）对源于具有不同营养缺陷型标记的拟康氏木霉菌株杂交的后代进行了分析。还对分生孢子、分生孢子发生和异核体的细胞学进行了研究。分析的大多数重组菌株的基因型与亲本菌株中的一个相似，而且多态性 RAPD 带的低频表明核融合可能不会发生在异核体中。在一些异核体区域，强烈染色的菌丝的存在可能与细胞死亡有关。我们提出可能发生了不同于有性生殖的体细胞重组机制，与融合后有限的营养体亲和性有关，这与其他拟康氏木霉物种的情况类似。
