Kremer E J, Kistler W S
Department of Chemistry, University of South Carolina, Columbia 29208.
Gene. 1992 Jan 15;110(2):167-73. doi: 10.1016/0378-1119(92)90644-5.
Gene H1t encodes a testis-specific variant of the H1 histone family expressed in pachytene spermatocytes during the meiotic phase of spermatogenesis. Fusions between various upstream fragments of the H1t gene and the chloramphenicol acetyltransferase-encoding reporter gene were analyzed in mouse L cells by both transient and permanent transfection. Expression of the minimal promoter [174 nucleotides (nt) upstream from the transcription start point] was enhanced by sequences extending to nt -693, but was reduced in constructs with kb of upstream sequence. Using synchronized cells, expression was at least twofold higher in growing than in inhibited cells. Thus, the H1t promoter is modulated both positively and negatively by distant upstream sequences, and it displays some of the S-phase-dependent character of a replication-dependent histone.
基因H1t编码H1组蛋白家族的一种睾丸特异性变体，在精子发生的减数分裂阶段于粗线期精母细胞中表达。通过瞬时转染和永久转染在小鼠L细胞中分析了H1t基因各种上游片段与编码氯霉素乙酰转移酶的报告基因之间的融合情况。最小启动子（转录起始点上游174个核苷酸）的表达在延伸至-693核苷酸的序列作用下增强，但在上游序列达千碱基的构建体中表达降低。使用同步化细胞，生长中的细胞中的表达比受抑制细胞中的表达至少高两倍。因此，H1t启动子受到远距离上游序列的正负调控，并且表现出一些依赖复制的组蛋白的S期依赖性特征。
