Drabent B, Doenecke D
Institut für Biochemie und Molekulare Zellbiologie, Abteilung Molekularbiologie, Georg-August-Universität, Göttingen, Germany.
Biol Reprod. 1997 Dec;57(6):1375-83. doi: 10.1095/biolreprod57.6.1375.
The testis-specific histone gene H1t is expressed only in mammalian testis at the stage of pachytene spermatocytes. The tissue-specific regulation of the mouse H1t gene was examined in mouse testicular primary culture cells with gene constructs consisting of H1t promoter elements fused to the chloramphenicol acetyltransferase or the firefly luciferase reporter gene. Our experiments demonstrate that expression of the mouse H1t gene is enhanced by a conserved H1 histone gene-specific TG box 452 base pairs upstream of the transcription start site. The transcription of the H1t gene appears to be reduced by sequences between -1999 and -1506. No regulatory effect could be shown for the H1 box in the expression of the mouse H1t gene. Binding of nuclear protein extracted from mouse testis to these consensus elements was shown by electrophoretic mobility-shift assays with mouse testicular nuclear proteins and labeled oligonucleotides containing the upstream TG box sequences or the two testis-specific elements of the H1t gene.
睾丸特异性组蛋白基因H1t仅在哺乳动物睾丸的粗线期精母细胞阶段表达。利用由与氯霉素乙酰转移酶或萤火虫荧光素酶报告基因融合的H1t启动子元件组成的基因构建体，在小鼠睾丸原代培养细胞中检测了小鼠H1t基因的组织特异性调控。我们的实验表明，小鼠H1t基因的表达在转录起始位点上游452个碱基对处的保守H1组蛋白基因特异性TG框的作用下增强。H1t基因的转录似乎在-1999至-1506之间的序列作用下减少。对于小鼠H1t基因的表达，未显示H1框有调控作用。通过用小鼠睾丸核蛋白和含有上游TG框序列或H1t基因的两个睾丸特异性元件的标记寡核苷酸进行电泳迁移率变动分析，显示从小鼠睾丸中提取的核蛋白与这些共有元件结合。
