运用随机扩增多态性DNA方法揭示肝细胞癌中的基因组不稳定性。
Genomic instability in hepatocellular carcinoma revealed by using the random amplified polymorphic DNA method.
作者信息
Zhang Shu-Hui, Cong Wen-Ming, Xian Zhi-Hong, Dong Hui, Wu Meng-Chao
机构信息
Department of Pathology, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, China.
出版信息
J Cancer Res Clin Oncol. 2004 Dec;130(12):757-61. doi: 10.1007/s00432-004-0613-4. Epub 2004 Sep 15.
PURPOSE
To investigate the genomic instability and their association with clinicopathological characteristics in hepatocellular carcinoma (HCC).
METHODS
DNA isolated from tumors and corresponding non-cancerous liver tissues of 56 patients with HCC was amplified with ten random 10-mer arbitrary primers by the random amplified polymorphic DNA (RAPD) method.
RESULTS
All the cases of HCC were demonstrated to have genomic instability by at least one primer. The incidence of genomic instability ranged from 20 to 70% in each case, and 17.9-50% in each primer. Serum AFP concentration, HBV infection, tumor size, histological grade, tumor capsule invasion, as well as intrahepatic metastasis were associated with the genomic instability on certain primers.
CONCLUSIONS
Genomic instability is a frequent event in HCC. The RAPD is an effective method for the identification and analysis of genomic instability in HCC, and it may provide new information for further evaluating the molecular mechanism of hepatocarcinogenesis.
目的
研究肝细胞癌（HCC）的基因组不稳定性及其与临床病理特征的关系。
方法
采用随机扩增多态性DNA（RAPD）方法，用10个随机的10聚体任意引物对56例HCC患者的肿瘤组织及相应的癌旁肝组织提取的DNA进行扩增。
结果
所有HCC病例至少有一个引物显示存在基因组不稳定。每个病例中基因组不稳定的发生率为20%至70%，每个引物的发生率为17.9%至50%。血清甲胎蛋白浓度、乙肝病毒感染、肿瘤大小、组织学分级、肿瘤包膜侵犯以及肝内转移与某些引物上的基因组不稳定有关。
结论
基因组不稳定在HCC中是常见事件。RAPD是鉴定和分析HCC基因组不稳定的有效方法，可为进一步评估肝癌发生的分子机制提供新信息。
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