Papadopoulos Sarantos, Benter Thomas, Anastassiou Gerasimos, Pape Michael, Gerhard Schaller, Bornfeld Norbert, Ludwig Wolf-Dieter, Dörken Bernd
Department of Obstetrics and Gynecology, Free University of Berlin, Berlin, Germany.
Int J Cancer. 2002 May 10;99(2):193-200. doi: 10.1002/ijc.10297.
Some types of cancer have been associated with abnormal DNA fingerprinting. We used random amplified polymorphic DNA (RAPD) to generate fingerprints that detect genomic alterations in human breast cancer. Primers were designed by choosing sequences involved in the development of DNA mutations. Seventeen primers in 44 different combinations were used to screen a total of 6 breast cancer DNA/normal DNA pairs and 6 uveal melanoma DNA/normal DNA pairs. Forty-five percent of these combinations reliably detected quantitative differences in the breast cancer pairs, while only 18% of these combinations detected differences in the uveal melanoma pairs. Fourteen (32%) and 12 (27%) primers generated a smear or did not produce any band patterns in the first and second cases, respectively. Taking into account the ability of RAPD to screen the whole genome, our results suggest that the genomic damage in breast cancer is significantly higher than in uveal melanoma. Our study confirms other reports that the molecular karyotypes produced with random priming, called amplotypes, are very useful for assessing genomic damage in cancer.
某些类型的癌症与异常的DNA指纹图谱有关。我们使用随机扩增多态性DNA（RAPD）来生成可检测人类乳腺癌基因组改变的指纹图谱。通过选择与DNA突变发生相关的序列来设计引物。使用17种引物的44种不同组合，总共筛选了6对乳腺癌DNA/正常DNA以及6对葡萄膜黑色素瘤DNA/正常DNA。这些组合中有45%能可靠地检测出乳腺癌配对中的定量差异，而在葡萄膜黑色素瘤配对中只有18%的组合能检测出差异。在第一种情况中，分别有14种（32%）引物产生了拖尾现象或未产生任何条带模式，在第二种情况中有12种（27%）引物如此。考虑到RAPD筛选全基因组的能力，我们的结果表明乳腺癌中的基因组损伤明显高于葡萄膜黑色素瘤。我们的研究证实了其他报告，即通过随机引物产生的分子核型（称为扩增型）对于评估癌症中的基因组损伤非常有用。
