Mulligan F J, Dillon P, Callan J J, Rath M, O'Mara F P
Department of Animal Husbandry and Production, Faculty of Veterinary Medicine, University College Dublin, Ireland.
J Dairy Sci. 2004 Oct;87(10):3451-60. doi: 10.3168/jds.S0022-0302(04)73480-3.
These experiments were designed to investigate nutritional means of reducing urine N excretion by grazing cows. In experiment 1, 36 Holstein-Friesian cows averaging 92 d in milk were fed either 1 or 6 kg of a high protein concentrate or 6 kg of a low protein concentrate. Pasture dry matter (DM) intake was higher for cows fed 1 kg of high protein concentrate (15.4 +/- 0.62 kg/d) than for cows fed 6 kg of low protein concentrate (13.4 +/- 0.55) but not for cows fed 6 kg of high protein concentrate (13.9 +/- 0.96). The reduction in pasture intake per kg of concentrate DM ingested amounted to 0.35 and 0.47 kg of pasture DM for cows fed 6 kg of high protein and 6 kg of low protein concentrate, respectively. Milk yield and milk protein yield were higher for cows fed 6 kg of high protein concentrate than for cows fed 1 kg of high protein concentrate. Cows fed 6 kg of high protein concentrate had the highest levels of N intake, total N excretion, and urine N excretion. The proportion of N excreted in the urine was lowest for cows fed 6 kg of low protein concentrate. Milk N excretion as a proportion of ingested N was higher for cows fed 6 kg of low protein concentrate than for cows fed 6 kg of high protein concentrate but not for cows fed 1 kg of high protein concentrate. In experiment 2, 24 Holstein-Friesian cows averaging 211 d in milk were supplemented with 4 kg of rolled barley or 4.32 kg of NaOH-treated barley. Milk yield and milk protein yield tended to be higher for cows fed rolled barley than for cows fed NaOH-treated barley. There was no difference in N intake, fecal N excretion, urinary N excretion, or milk N output between diets. Milk urea N concentration was lower for cows fed rolled barley. Significant positive linear relationships were found between N intake and fecal N excretion, urine N excretion, and milk N excretion in experiment 1. In experiment 2, the relationships between N intake and fecal N excretion and urine N excretion were curvilinear, with urine N excretion increasing at a decreasing rate, and fecal N excretion increasing at an increasing rate, as N intake increased. The N excreted by dairy cows may be partitioned to fecal N if supplements based on high concentrations of fermentable organic matter and low concentrations of N are fed. Refinement of this nutritional strategy may allow reduced N excretion without reducing animal performance.
这些实验旨在研究通过营养手段减少放牧奶牛尿液氮排泄量。在实验1中,选取36头平均产奶92天的荷斯坦 - 弗里生奶牛,分别给它们饲喂1千克或6千克高蛋白浓缩饲料,或者6千克低蛋白浓缩饲料。采食1千克高蛋白浓缩饲料的奶牛的牧场干物质摄入量(15.4±0.62千克/天)高于采食6千克低蛋白浓缩饲料的奶牛(13.4±0.55千克/天),但与采食6千克高蛋白浓缩饲料的奶牛(13.9±0.96千克/天)无差异。每摄入1千克浓缩饲料干物质,采食6千克高蛋白和6千克低蛋白浓缩饲料的奶牛的牧场采食量分别减少0.35千克和0.47千克牧场干物质。采食6千克高蛋白浓缩饲料的奶牛的产奶量和乳蛋白产量高于采食1千克高蛋白浓缩饲料的奶牛。采食6千克高蛋白浓缩饲料的奶牛的氮摄入量、总氮排泄量和尿液氮排泄量最高。采食6千克低蛋白浓缩饲料的奶牛尿液中排出的氮的比例最低。采食6千克低蛋白浓缩饲料的奶牛的乳氮排泄量占摄入氮的比例高于采食6千克高蛋白浓缩饲料的奶牛,但与采食1千克高蛋白浓缩饲料的奶牛无差异。在实验2中,选取24头平均产奶211天的荷斯坦 - 弗里生奶牛,分别给它们补饲4千克碾压大麦或4.32千克氢氧化钠处理的大麦。采食碾压大麦的奶牛的产奶量和乳蛋白产量往往高于采食氢氧化钠处理大麦的奶牛。不同日粮之间的氮摄入量、粪便氮排泄量、尿液氮排泄量或乳氮产量没有差异。采食碾压大麦的奶牛的乳尿素氮浓度较低。在实验1中,发现氮摄入量与粪便氮排泄量、尿液氮排泄量和乳氮排泄量之间存在显著的正线性关系。在实验2中,氮摄入量与粪便氮排泄量和尿液氮排泄量之间的关系呈曲线,随着氮摄入量增加,尿液氮排泄量以递减速率增加,粪便氮排泄量以递增速率增加。如果饲喂基于高浓度可发酵有机物和低浓度氮的补充料,奶牛排出的氮可能会分配到粪便氮中。优化这种营养策略可能在不降低动物生产性能的情况下减少氮排泄。