Galo E, Emanuele S M, Sniffen C J, White J H, Knapp J R
Department of Animal Science, University of Vermont, Burlington 05405, USA.
J Dairy Sci. 2003 Jun;86(6):2154-62. doi: 10.3168/jds.S0022-0302(03)73805-3.
The purpose of this study was to evaluate the impact of polymer-coated urea on nitrogen retention, rumen microbial growth, and milk production and composition. Coated urea (CU) that is more slowly hydrolyzed to ammonia than unprotected urea could potentially be used more efficiently by rumen microorganisms. Eight cows were offered each of three diets in a randomized crossover design. Each treatment period consisted of a 14-d adjustment period and a 5-d collection period. Diets were formulated to maintain milk production while reducing plasma urea nitrogen concentrations and urinary nitrogen excretion. Diets consisted of corn silage, mixed grass/legume haylage, chopped alfalfa hay, corn meal, protein, vitamin and mineral supplements, in a total mixed ration and fed ad libitum. The diets contained 17.9%, 18.1%, and 16.4% CP and 0, 0.77%, and 0.77% CU (dry matter basis) and are denoted as CP18-CU, CP18+CU, and CP16+CU, respectively. Individual feed intakes were measured, and total fecal, and urine collections were conducted. Cows were milked twice daily at 0500 and 1700 h, and the milk sampled for composition and milk urea N analysis. Dry matter intake averaged 23.5 +/- 0.2 kg/d and was not altered by diet. Also, milk fat and true protein were not altered by diet and averaged 3.72 and 3.07%, respectively. Milk yield was highest for diets CP18-CU and CP18+CU. Significant differences were observed in N intake and excretion in urine, feces, and milk between dietary treatments. Cows fed CP16+CU consumed 11% less N than in CP18-CU. Cows fed CP18+CU showed the highest excretion of N in urine, and together with CP16+CU, the lowest N excretion in feces. Nitrogen excretion in milk was lower for cows fed CP16+CU. Calculated N balance was not significantly different between diets nor was it significantly different from zero. Efficiency of N capture in milk protein as a function of N intake was higher for animals on CP16+CU. Urinary excretion of purine derivatives was not different between diets, and estimated microbial CP was also similar. Coated urea was not effective at reducing nitrogen excretion by dairy cattle.
本研究的目的是评估聚合物包被尿素对氮保留、瘤胃微生物生长以及牛奶产量和成分的影响。与未保护的尿素相比,水解成氨的速度更慢的包被尿素(CU)可能会被瘤胃微生物更有效地利用。采用随机交叉设计,给8头奶牛分别投喂三种日粮中的每一种。每个处理期包括一个14天的调整期和一个5天的收集期。日粮的配方旨在维持牛奶产量,同时降低血浆尿素氮浓度和尿氮排泄量。日粮由玉米青贮、混合禾本科/豆科青贮料、切碎的苜蓿干草、玉米粉、蛋白质、维生素和矿物质补充剂组成,制成全混合日粮,自由采食。日粮的粗蛋白含量分别为17.9%、18.1%和16.4%,CU含量分别为0、0.77%和0.77%(干物质基础),分别记为CP18-CU、CP18+CU和CP16+CU。测量个体采食量,并进行粪便和尿液的总收集。奶牛每天0500和1700时挤奶两次,并采集牛奶样本进行成分分析和牛奶尿素氮分析。干物质采食量平均为23.5±0.2 kg/d,不受日粮影响。此外,牛奶脂肪和真蛋白不受日粮影响,平均分别为3.72%和3.07%。CP18-CU和CP18+CU日粮的牛奶产量最高。不同日粮处理之间在氮摄入量以及尿液、粪便和牛奶中的氮排泄量上存在显著差异。饲喂CP16+CU的奶牛比饲喂CP18-CU的奶牛少摄入11%的氮。饲喂CP18+CU的奶牛尿液中的氮排泄量最高,与CP16+CU一起,粪便中的氮排泄量最低。饲喂CP16+CU的奶牛牛奶中的氮排泄量较低。计算得出的氮平衡在不同日粮之间没有显著差异,也与零没有显著差异。CP16+CU日粮组动物的牛奶蛋白中氮捕获效率作为氮摄入量的函数更高。不同日粮之间嘌呤衍生物的尿排泄量没有差异,估计的微生物粗蛋白也相似。包被尿素对减少奶牛的氮排泄无效。