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系统性微小RNA组分析揭示了微小RNA在乳蛋白代谢和品质中的作用:对低质饲草利用的见解

Systematic microRNAome profiling reveals the roles of microRNAs in milk protein metabolism and quality: insights on low-quality forage utilization.

作者信息

Wang Diming, Liang Guanxiang, Wang Bing, Sun Huizeng, Liu Jianxin, Guan Le Luo

机构信息

Institute of Dairy Sciences, College of Animal Sciences, Zhejiang University, Hangzhou, P R, China.

Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada.

出版信息

Sci Rep. 2016 Feb 17;6:21194. doi: 10.1038/srep21194.

Abstract

In this study, we investigated the molecular regulatory mechanisms of milk protein production in dairy cows by studying the miRNAomes of five key metabolic tissues involved in protein synthesis and metabolism from dairy cows fed high- and low-quality diets. In total, 340, 338, 337, 330, and 328 miRNAs were expressed in the rumen, duodenum, jejunum, liver, and mammary gland tissues, respectively. Some miRNAs were highly correlated with feed and nitrogen efficiency, with target genes involved in transportation and phosphorylation of amino acid (AA). Additionally, low-quality forage diets (corn stover and rice straw) influenced the expression of feed and nitrogen efficiency-associated miRNAs such as miR-99b in rumen, miR-2336 in duodenum, miR-652 in jejunum, miR-1 in liver, and miR-181a in mammary gland. Ruminal miR-21-3p and liver miR-2285f were predicted to regulate AA transportation by targeting ATP1A2 and SLC7A8, respectively. Furthermore, bovine-specific miRNAs regulated the proliferation and morphology of rumen epithelium, as well as the metabolism of liver lipids and branched-chain AAs, revealing bovine-specific mechanisms. Our results suggest that miRNAs expressed in these five tissues play roles in regulating transportation of AA for downstream milk production, which is an important mechanism that may be associated with low milk protein under low-quality forage feed.

摘要

在本研究中,我们通过研究采食优质和劣质日粮的奶牛参与蛋白质合成与代谢的五个关键代谢组织的微小RNA组,来探究奶牛乳蛋白产生的分子调控机制。瘤胃、十二指肠、空肠、肝脏和乳腺组织中分别表达了340、338、337、330和328个微小RNA。一些微小RNA与饲料和氮效率高度相关,其靶基因参与氨基酸(AA)的转运和磷酸化。此外,劣质粗饲料日粮(玉米秸秆和稻草)影响了与饲料和氮效率相关的微小RNA的表达,如瘤胃中的miR-99b、十二指肠中的miR-2336、空肠中的miR-652、肝脏中的miR-1以及乳腺中的miR-181a。瘤胃中的miR-21-3p和肝脏中的miR-2285f分别通过靶向ATP1A2和SLC7A8来调控AA转运。此外,牛特异性微小RNA调控瘤胃上皮细胞的增殖和形态,以及肝脏脂质和支链氨基酸的代谢,揭示了牛特异性机制。我们的结果表明,在这五个组织中表达的微小RNA在调节AA转运以促进下游乳蛋白产生中发挥作用,这是一种可能与劣质粗饲料喂养下乳蛋白含量低相关的重要机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbad/4756660/6b52c6727d0d/srep21194-f1.jpg

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