大蒜素可抑制肠道上皮细胞中促炎细胞因子和趋化因子的自发分泌以及由肿瘤坏死因子-α诱导的分泌。

Allicin inhibits spontaneous and TNF-alpha induced secretion of proinflammatory cytokines and chemokines from intestinal epithelial cells.

作者信息

Lang Alon, Lahav Maor, Sakhnini Emad, Barshack Iris, Fidder Herma H, Avidan Benjamin, Bardan Eitan, Hershkoviz Rami, Bar-Meir Simon, Chowers Yehuda

机构信息

Department of Gastroenterology, Chaim Sheba Medical Center, Affiliaated to the Tel-Aviv University, Tel-Hashomer 5261, Israel.

出版信息

Clin Nutr. 2004 Oct;23(5):1199-208. doi: 10.1016/j.clnu.2004.03.011.

DOI:10.1016/j.clnu.2004.03.011

PMID:15380914

Abstract

BACKGROUND & AIMS: Allicin, the active substance of fresh crushed garlic has different biological activities and was implicated as an anti-inflammatory agent. Epithelial cells have an important role in intestinal inflammation. The aim of this study was to assess the immunomodulatory effect of allicin on intestinal epithelial cells.

METHODS

The spontaneous and TNF-alpha-stimulated secretion of IL-1beta, IL-8, IP-10 and MIG from HT-29 and Caco-2 cells was tested with, or without pretreatment with allicin. Cytokine secretion was assessed using ELISA and expression of mRNA was determined by an RNA protection assay.

RESULTS

Allicin markedly inhibited the spontaneous and TNF-alpha -induced secretion of IL-1beta, IL-8, IP-10 and MIG from the two different cell lines in a dose-dependent manner and suppressed the expression of IL-8 and IL-1beta mRNA levels. In addition, allicin suppressed the degradation of IkappaB. No effect on cell viability was noted.

CONCLUSIONS

These observations indicate that allicin exerts an inhibitory immunomodulatory effect on intestinal epithelial cells and suggest that allicin may have the potential to attenuate intestinal inflammation.

摘要

背景与目的

大蒜素是新鲜碾碎大蒜的活性物质，具有多种生物学活性，被认为是一种抗炎剂。上皮细胞在肠道炎症中起重要作用。本研究旨在评估大蒜素对肠上皮细胞的免疫调节作用。

方法

用或不用大蒜素预处理HT-29和Caco-2细胞，检测其自发分泌以及肿瘤坏死因子-α（TNF-α）刺激分泌白细胞介素-1β（IL-1β）、白细胞介素-8（IL-8）、干扰素诱导蛋白10（IP-10）和γ干扰素诱导的单核因子（MIG）的情况。采用酶联免疫吸附测定法（ELISA）评估细胞因子分泌情况，通过RNA保护试验测定mRNA表达。