Abdel-latief Mohatmed, Meyering-Vos Martina, Hoffmann Klaus H
Department of Animal Ecology I, University of Bayreuth, Universitaetsstrasse 30, 95440, Bayreuth, Germany.
Regul Pept. 2004 Oct 15;122(2):69-78. doi: 10.1016/j.regpep.2004.05.015.
A cDNA that encodes 53 amino acids, including one copy of the RVRGNPISCF-OH peptide, was cloned from Spodoptera frugiperda. This peptide strongly stimulates the synthesis and release of juvenile hormone (JH) in vitro by the corpora allata (CA) of S. frugiperda and was code-named Spofr-AT 2. Northern blotting and reverse transcriptase polymerase chain reaction (RT-PCR) analyses revealed that the preprohormone is expressed as one transcript in the brain, midgut (Mg) and ovary (Ov) in a tissue- and developmental-specific manner. Whole-mount in situ hybridization confirmed the gene expression in the suboesophageal ganglion (SOG) and in the ovary of adult females. Treating the CA with the synthetic peptide caused an up to tenfold increase in the release of JH. The stimulation was dose-dependent with an apparent EC(50) of ca. 10(-7) M. CA that were activated with Spofr-AT 2 could be inhibited by the addition of synthetic allatostatin type-C from Manduca sexta (Manse-AS). This is the first report on the presence and function of two different peptides with allatotropic activity in an insect species.
从草地贪夜蛾中克隆出一个编码53个氨基酸的cDNA，其中包括一个RVRGNPISCF - OH肽拷贝。该肽在体外能强烈刺激草地贪夜蛾咽侧体（CA）合成和释放保幼激素（JH），并被命名为Spofr - AT 2。Northern印迹法和逆转录聚合酶链反应（RT - PCR）分析表明，前激素原以组织和发育特异性的方式在脑、中肠（Mg）和卵巢（Ov）中作为一种转录本表达。整装原位杂交证实了该基因在咽下神经节（SOG）和成年雌性卵巢中的表达。用合成肽处理CA会使JH释放量增加多达10倍。这种刺激是剂量依赖性的，表观EC(50)约为10(-7) M。用Spofr - AT 2激活的CA可被添加来自烟草天蛾的合成C型咽侧体抑制素（Manse - AS）所抑制。这是关于昆虫物种中具有促咽侧体活性的两种不同肽的存在和功能的首次报道。
