Abdel-Latief Mohatmed, Meyering-Vos Martina, Hoffmann Klaus H
Department of Animal Ecology I, University of Bayreuth, Bayreuth, Germany.
Arch Insect Biochem Physiol. 2004 Jul;56(3):120-32. doi: 10.1002/arch.20003.
The gene encoding the Spodoptera frugiperda allatostatin type-A peptide family (Y/FXFGL-amides) was isolated from S. frugiperda brain cDNA. The gene encodes a precursor of 231 amino acids containing nine (or ten) Y/FXFGL-a peptides that are tandemly arranged in three blocks. The comparison of the Spofr-AST A precursor with the respective precursor genes from two other lepidopteran species, Helicoverpa armigera and Bombyx mori, shows high homology in size, sequence (84 and 57%, respectively), and organisation of the allatostatins. One-step RT-PCR analysis using a Spofr-AST A-6 to A-9 probe shows that the gene is not only expressed as one transcript in the brain and midgut of larvae and adults in a time- and tissue-specific manner, but also in the reproductive tissues of adult S. frugiperda. Data confirm the nature of the allatostatin type-A peptides as brain/gut myoregulatory hormones, whereas their function(s) in ovaries, oviduct, and testes still have to be resolved. The cell-specific localization of the preprohormone expression, as demonstrated by whole mount in situ hybridization, confirms the overall distribution of the Spofr-AST A preprohormone as shown by RT-PCR and supports the pleiotropic functions of the peptides.
从草地贪夜蛾脑cDNA中分离出编码草地贪夜蛾A 型咽侧体抑制素肽家族（Y/FXFGL-酰胺）的基因。该基因编码一个由231个氨基酸组成的前体，其中包含九个（或十个）Y/FXFGL-a肽，这些肽串联排列成三个区域。将草地贪夜蛾A 型咽侧体抑制素前体与另外两种鳞翅目昆虫棉铃虫和家蚕的相应前体基因进行比较，结果表明，它们在大小、序列（分别为84%和57%）以及咽侧体抑制素的组织方式上具有高度同源性。使用草地贪夜蛾A 型咽侧体抑制素A-6至A-9探针进行的一步RT-PCR分析表明，该基因不仅在幼虫和成虫的脑和中肠中以时间和组织特异性方式作为一种转录本表达，而且在成年草地贪夜蛾的生殖组织中也有表达。数据证实了A 型咽侧体抑制素肽作为脑/肠肌调节激素的性质，而它们在卵巢、输卵管和睾丸中的功能仍有待确定。通过整体原位杂交证明的前激素原表达的细胞特异性定位，证实了RT-PCR所示的草地贪夜蛾A 型咽侧体抑制素前激素原的整体分布，并支持了这些肽的多效性功能。
