Zhang Xiangru, Minear Roger A, Guo Yingbo, Hwang Cordelia J, Barrett Sylvia E, Ikeda Kazuhiro, Shimizu Yoshihisa, Matsui Saburo
Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Water Res. 2004 Nov;38(18):3920-30. doi: 10.1016/j.watres.2004.06.022.
Identification of chlorinated drinking water disinfection byproducts (DBPs) was investigated by using electrospray ionization-mass spectrometry/mass spectrometry (ESI-MS/MS). Chlorine-containing compounds were found to form chloride ion fragments by MS/MS, which can be used as a 'fingerprint' for chlorinated DBPs. Instrumental parameters that affect the formation of chloride ions by ESI-MS/MS were examined, and appropriate conditions for use in finding specific structural information were evaluated. The results show that maximizing the formation of chloride ions by MS/MS required a relatively high collision energy and collision gas pressure; also, limiting the scan range to m/z 30-40 allowed improved sensitivity for detection; but obtaining structural information required the use of lower collision energies. The conditions obtained were demonstrated to be effective in identifying chlorinated DBPs in a standard sample with relatively low concentrations of each component and in a chlorinated humic substance sample. Sample pretreatment techniques including ultrafiltration and size exclusion chromatography appeared to be helpful for identifying highly polar or high molecular weight chlorine-containing DBPs by ESI-MS/MS.
采用电喷雾电离-质谱/质谱（ESI-MS/MS）对氯化饮用水消毒副产物（DBPs）进行了鉴定研究。发现含氯化合物通过MS/MS形成氯离子碎片，可作为氯化DBPs的“指纹”。研究了影响ESI-MS/MS形成氯离子的仪器参数，并评估了用于寻找特定结构信息的合适条件。结果表明，通过MS/MS使氯离子形成最大化需要相对较高的碰撞能量和碰撞气体压力；此外，将扫描范围限制在m/z 30-40可提高检测灵敏度；但获得结构信息需要使用较低的碰撞能量。所获得的条件被证明在鉴定各组分浓度相对较低的标准样品和氯化腐殖质样品中的氯化DBPs时是有效的。包括超滤和尺寸排阻色谱在内的样品预处理技术似乎有助于通过ESI-MS/MS鉴定高极性或高分子量的含氯DBPs。
