Deshpande Shripad B, Gupta Narendra K, Shukla Vijay K
Department of Physiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.
Dig Dis Sci. 2004 Aug;49(7-8):1342-7. doi: 10.1023/b:ddas.0000037832.44729.a7.
The present investigation was undertaken to delineate the in vitro responsiveness of cholelithiatic gallbladders to cholecystokinin (CCK) and compared with those evoked by carbachol, histamine, or electrical stimulation. Gallbladder muscular strips (2-3 mm wide and 15-20 mm long) from patients undergoing cholecystectomy were used for recording the in vitro contractions evoked by electrical and chemical (carbachol, histamine, or cholecystokinin) stimulation. Stimulation of strips with trains of pulses (5-msec duration, 70 V at 100 Hz) of varying train durations (0.01 to 9 sec) elicited duration-dependent increase in the amplitude of contractions and the maximal contractions were seen with 5 sec. Atropine (0.4 microM) significantly attenuated these contractions, leaving about 34% of contractions, which in turn was abolished by xylocaine. Carbachol produced a concentration-dependent (0.004-0.4 microM) increase in force of contraction and the maximal response was seen at 0.4 microM and abolished by atropine (0.4 microM). Histamine also produced contractions and the maximal contractions were about 35% of the maximal carbachol response. Histamine-induced contractions were not abolished by atropine but were abolished by xylocaine. CCK up to 10 microM failed to evoke any contraction, even though the strips were responsive to carbachol. The results indicate that cholelithiatic gallbladders exhibited responses to electrical stimulation through cholinergic and histaminergic plexuses and they were insensitive to CCK.
本研究旨在描述胆石症胆囊在体外对胆囊收缩素（CCK）的反应性，并与卡巴胆碱、组胺或电刺激所诱发的反应进行比较。取自接受胆囊切除术患者的胆囊肌条（宽2 - 3毫米，长15 - 20毫米）用于记录电刺激和化学刺激（卡巴胆碱、组胺或胆囊收缩素）诱发的体外收缩。用不同串长（0.01至9秒）的脉冲串（持续时间5毫秒，100赫兹时70伏）刺激肌条，可引起收缩幅度随串长增加，5秒时出现最大收缩。阿托品（0.4微摩尔）显著减弱这些收缩，使收缩幅度降至约34%，而这又被利多卡因消除。卡巴胆碱使收缩力呈浓度依赖性增加（0.004 - 0.4微摩尔），0.4微摩尔时出现最大反应，且被阿托品（0.4微摩尔）消除。组胺也可引起收缩，最大收缩幅度约为卡巴胆碱最大反应的35%。组胺诱发的收缩不被阿托品消除，但被利多卡因消除。高达10微摩尔的CCK未能诱发任何收缩，尽管肌条对卡巴胆碱有反应。结果表明，胆石症胆囊通过胆碱能和组胺能神经丛对电刺激有反应，而对CCK不敏感。
