Parkman H P, James A N, Thomas R M, Bartula L L, Ryan J P, Myers S I
Department of Medicine, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA.
J Surg Res. 2001 Mar;96(1):135-42. doi: 10.1006/jsre.2001.6082.
The aim of this study was to determine whether the prostaglandin synthase inhibitor indomethacin reverses the inflammation and abnormal gallbladder contractility that occur after common bile duct ligation (CBDL), a model of acute cholecystitis.
Gallbladder muscle contractility was studied in vitro in normal, CBDL, and sham-operated guinea pigs. Animals were treated with saline or indomethacin in vivo. Acetylcholine (ACh) was used to directly contract the muscle and electric field stimulation (EFS) to activate intrinsic nerves. Hematoxylin and eosin-stained slides of muscle strips were scored for inflammation.
CBDL in saline-treated animals increased the inflammation score and decreased gallbladder muscle contractility to ACh and EFS. Indomethacin decreased the inflammation score and partly reversed the smooth muscle contractile response to ACh 6 and 24 h after CBDL, but not at 48 h. Indomethacin did not reverse the CBDL-induced decrease in nerve-evoked contractions.
Gallbladder inflammation and contractile dysfunction after CBDL are partly reversed with indomethacin at 6 and 24 h, but not at 48 h. This suggests that, early in the course of CBDL, the inflammation and contractile dysfunction are, in part, prostaglandin-mediated.
本研究旨在确定前列腺素合成酶抑制剂吲哚美辛是否能逆转急性胆囊炎模型——胆总管结扎（CBDL）后出现的炎症和胆囊收缩异常。
在正常、CBDL和假手术的豚鼠体内对胆囊肌肉收缩性进行体外研究。动物在体内接受生理盐水或吲哚美辛治疗。使用乙酰胆碱（ACh）直接使肌肉收缩，并用电场刺激（EFS）激活内在神经。对苏木精和伊红染色的肌条玻片进行炎症评分。
在接受生理盐水治疗的动物中，CBDL增加了炎症评分，并降低了胆囊肌肉对ACh和EFS的收缩性。吲哚美辛降低了炎症评分，并在CBDL后6小时和24小时部分逆转了平滑肌对ACh的收缩反应，但在48小时未逆转。吲哚美辛并未逆转CBDL诱导的神经诱发收缩的降低。
CBDL后胆囊炎症和收缩功能障碍在6小时和24小时时部分被吲哚美辛逆转，但在48小时时未被逆转。这表明，在CBDL病程早期，炎症和收缩功能障碍部分是由前列腺素介导的。
